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Tissue culture and quick propagation method of potamogeton distinctus dormant buds

A technology of tissue culture and dormant buds, which is applied in the field of plant tissue culture, can solve the problems of easily broken stems and difficult collection of medicinal materials, and achieve the effect of promoting cell differentiation and division, and promoting the formation of

Active Publication Date: 2020-09-08
VOCATIONAL & TECHN COLLEGE OF ANSHUN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The whole herb is also used as medicine, and the stems are easy to break when collected, so it is difficult to collect a large amount of medicinal materials

Method used

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  • Tissue culture and quick propagation method of potamogeton distinctus dormant buds
  • Tissue culture and quick propagation method of potamogeton distinctus dormant buds
  • Tissue culture and quick propagation method of potamogeton distinctus dormant buds

Examples

Experimental program
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Effect test

Embodiment 1

[0036] A method for tissue culture and rapid propagation of dormant buds of Sage chinensis, comprising the following steps:

[0037] (1) Collection and pretreatment of explants: select the dormant buds of Sclerophylla sativa as explants, wash them directly with tap water for 1 hour, and filter them dry with sterile gauze; then place them on a sterile operating table, Wipe once with sterile absorbent cotton soaked in 75% ethanol, remove excess bud scales, adventitious roots and other attachments, then use a sterile scalpel to cut off clustered dormant buds individually from the base, and use sterile water to cut off individual dormant buds repeatedly Rinse 4 to 5 times before proceeding to the next step;

[0038] (2) Disinfection treatment: Put the dormant buds of S. argyi obtained in step (1) on a sterile operating table and soak them in 75% alcohol for 30-40 seconds, then soak them in 0.1% mercury liter for 10 minutes, take them out Finally, use sterile water to repeatedly r...

Embodiment 2

[0044] A method for tissue culture and rapid propagation of dormant buds of Sage chinensis, comprising the following steps:

[0045] (1) Collection and pretreatment of explants: select the dormant buds of Sclerophylla sativa as explants, wash them directly with tap water for 1 hour, and filter them dry with sterile gauze; then place them on a sterile operating table, Wipe once with sterile absorbent cotton soaked in 75% ethanol, remove excess bud scales, adventitious roots and other attachments, then use a sterile scalpel to cut off clustered dormant buds individually from the base, and use sterile water to cut off individual dormant buds repeatedly Rinse 4 to 5 times before proceeding to the next step;

[0046] (2) Disinfection treatment: Put the dormant buds of S. argyi obtained in step (1) on a sterile operating table and soak them in 75% alcohol for 30-40 seconds, then soak them in 0.1% mercury liter for 10 minutes, take them out Finally, use sterile water to repeatedly r...

Embodiment 3

[0052] A method for tissue culture and rapid propagation of dormant buds of Sage chinensis, comprising the following steps:

[0053] (1) Collection and pretreatment of explants: select the dormant buds of Sclerophylla sativa as explants, wash them directly with tap water for 1 hour, and filter them dry with sterile gauze; then place them on a sterile operating table, Wipe once with sterile absorbent cotton soaked in 75% ethanol, remove excess bud scales, adventitious roots and other attachments, then use a sterile scalpel to cut off clustered dormant buds individually from the base, and use sterile water to cut off individual dormant buds repeatedly Rinse 4 to 5 times before proceeding to the next step;

[0054] (2) Disinfection treatment: Put the dormant buds of S. argyi obtained in step (1) on a sterile operating table and soak them in 75% alcohol for 30-40 seconds, then soak them in 0.1% mercury liter for 10 minutes, take them out Finally, use sterile water to repeatedly r...

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Abstract

The invention discloses a tissue culture and quick propagation method of potamogeton distinctus dormant buds. The tissue culture and quick propagation method of potamogeton distinctus dormant buds comprises the following steps (1) performing explant collection and pretreatment; (2) performing disinfection treatment; (3) performing adventitious bud induction; (4) performing propagation culture; (5)performing rooting culture; and (6) performing seedling hardening and transplanting. The method disclosed by the invention can be free from influence of seasons, temperature and regions, and massivepotamogeton distinctus bacteria-free seedlings can be produced; a bacteria-free propagation system of the potamogeton distinctus dormant buds is established, and a theoretical basis and technical support are provided for directly cultivating potamogeton distinctus medical locations in laboratories subsequently; and in addition, the tissue culture and quick propagation method also has important significance in systematically researching the potamogeton distinctus, protecting and repairing wetlands and repairing ecological environment.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of dormant buds of Sage chinensis. Background technique [0002] Eyed vegetables ( Potamogeton distinctus A. Bennett J. Bot.) is a perennial aquatic herb of Potamogetonaceae (Potamogetonaceae), with well-developed rhizomes, white, 1.5-2 mm in diameter, multi-branched, and often form a spindle-shaped dormancy at the top Buds with slightly dense fibrous roots at nodes. Stems terete, 1.5-2 mm in diam., usually unbranched. Floating leaves leathery, lanceolate, broadly lanceolate to ovate-lanceolate, 2-10 cm long, 1-4 cm wide, apex acute or obtusely rounded, base obtusely rounded or sometimes nearly cuneate, 5-20 cm long The stipe; the leaf veins are many, the top is connected; the submerged leaves are lanceolate to narrowly lanceolate, herbaceous, with stipe, often falling early; the stipules are membranous, 2-7 cm lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008Y02A40/22
Inventor 宋伦洪开文陈懿郑重陶金宋泽刘晶许锋
Owner VOCATIONAL & TECHN COLLEGE OF ANSHUN
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