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Microfluidic structures for plasma separation and detection

A plasma separation and microfluidic technology, applied in fluid controllers, laboratory containers, chemical instruments and methods, etc., can solve the problems of complex separation process, time-consuming, high cost, etc., to improve detection efficiency and simplify experiments The effect of the operation

Active Publication Date: 2020-11-06
TIANJIN DEXIANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods either require a large blood collection volume (for example, more than a few milliliters), or the separation process is complicated and time-consuming, or the cost is high (for example, membrane filtration)

Method used

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  • Microfluidic structures for plasma separation and detection
  • Microfluidic structures for plasma separation and detection
  • Microfluidic structures for plasma separation and detection

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Embodiment

[0037] The upper and lower layers of the microfluidic structure are prepared by injection molding using polycarbonate (PC), and the upper and lower layers are bonded to form the final shape. Before bonding, use isopropanol to dilute Hydro 300 (Shanghai Sangjing Chemical Co., Ltd.) to 30% concentration, apply it evenly on the right side of the U-shaped channel (forming a hydrophilic area), the reaction area channel and the reaction area, and dry it in a ventilated place Dry for 2 hours, then bond the upper and lower layers to form a complete microfluidic structure.

[0038] The inner diameter of the U-shaped channel is 1.5mm, the total length is 30mm, the length on both sides is 15mm, and the inner diameter of the channel in the reaction zone is 0.5mm. The volume of the sample tank is 30 μL. When in use, add 30 μL whole blood sample from the sampling port, after centrifugation at 700gx2min, the plasma is separated from the whole blood at the lower part of the U-shaped channel,...

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Abstract

Provided herein is a microfluidic structure for plasma separation and detection that employs a U-shaped channel having a partially hydrophobic inner surface and a partially hydrophilic inner surface to achieve the purpose of separating plasma from a blood sample by centrifugation and using the separated plasma directly for detection.

Description

technical field [0001] The invention relates to a microfluidic structure, especially a microfluidic structure that can be used for plasma separation and detection. Background technique [0002] Clinically, the method of detecting the concentration or existence of certain biological components (such as antibodies) in blood is often used for blood group analysis, disease diagnosis, drug efficacy determination, etc. Most of these methods require the use of blood cell-depleted plasma or serum to exclude the influence of blood cells on the detection process. For example, the presence of red blood cells in whole blood often makes detection impossible for some methods that require a color change to determine the concentration of the analyte in the sample. As another example, for the reverse typing of blood group through cell agglutination reaction, it is obviously necessary to remove the red blood cells in the blood to be tested. Therefore, in clinical testing, it is first necess...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00
CPCB01L3/5027B01L2200/10B01L2300/0861B01L2300/0887B01L2300/12B01L2300/161B01L2400/0409
Inventor 王丽黄志刚王秀柱王伟权王亚强
Owner TIANJIN DEXIANG BIOTECHNOLOGY CO LTD