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Extraction method of microsporidium DNA based on 3D printing functive, reagent kit and application of extraction method

A 3D printing and microsporidia technology, which is applied in the cleaning method using liquid, DNA preparation, recombinant DNA technology, etc. Extraction method is quick and easy to operate

Pending Publication Date: 2020-09-11
LIAONING OCEAN & FISHERIES SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the cumbersome and complicated extraction process of microsporidia nucleic acid and low throughput. The present invention firstly provides a method for extracting microsporidian DNA based on 3D printed special-shaped functional body, which includes the following steps:

Method used

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  • Extraction method of microsporidium DNA based on 3D printing functive, reagent kit and application of extraction method
  • Extraction method of microsporidium DNA based on 3D printing functive, reagent kit and application of extraction method
  • Extraction method of microsporidium DNA based on 3D printing functive, reagent kit and application of extraction method

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Experimental program
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Embodiment 1

[0059] Using photosensitive resin (PAA) as raw material, using DLP digital image projection 3D printing technology to undergo photocuring reaction under 400-800nm ​​light, so as to make such figure 1 The 3D printed special-shaped functional body with a regular structure shown in b: the 3D printed special-shaped functional body is a structure similar to an umbrella, and the eight-unit 3D printed special-shaped functional body is composed of a nucleic acid binding area 1, a handle area 2 and a connecting area 3. The nucleic acid binding area 1 is a cone, the handle area 2 is a cylinder, and the connection area 3 is a long strip. The center of the cone bottom of the nucleic acid binding area 1 is connected to one bottom surface of the handle area 2, and the other bottom surface of the handle area 2 is connected to the connection area. 3 connection, connect 8 single 3D printing special-shaped functional bodies side by side on the connection area 3 to form eight 3D printing special-...

Embodiment 2

[0066] Using photosensitive resin (PAA) as raw material, using DLP digital image projection 3D printing technology to undergo photocuring reaction under 400-800nm ​​light, so as to make such figure 1 The 3D printed special-shaped functional body with the regular structure shown in a: the 3D printed special-shaped functional body is similar to an umbrella structure, and the single 3D printed special-shaped functional body is composed of a nucleic acid binding area 1 and a handle area 2, and the nucleic acid binding area 1 is a cone body, the handle area 2 is a cylinder, and the center of the cone bottom of the nucleic acid binding area 1 is connected to a bottom surface of the handle area 2. Specifically, it includes one monomer functional body with a single nucleic acid binding region 1, the nucleic acid binding region 1 has a width of 5mm and a height of 10mm, the outer surface of the nucleic acid binding region 1 has a thread and groove structure, and the handle region 2 has ...

Embodiment 3

[0070] Using photosensitive resin (PAA) as raw material, using DLP digital image projection 3D printing technology to undergo photocuring reaction under fixed wavelength ultraviolet light, so as to make such figure 1 The 3D printed special-shaped functional body with the regular structure shown in a: the 3D printed special-shaped functional body is similar to an umbrella structure, and the single 3D printed special-shaped functional body is composed of a nucleic acid binding area 1 and a handle area 2, and the nucleic acid binding area 1 is a cone body, the handle area 2 is a cylinder, and the center of the cone bottom of the nucleic acid binding area 1 is connected to a bottom surface of the handle area 2. Specifically, it includes 2 monomer functional bodies with a single nucleic acid binding region 1. The nucleic acid binding region 1 has a width of 5mm and a height of 10mm. The outer surface of the nucleic acid binding region 1 has a thread and groove structure, and the han...

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Abstract

The invention discloses an extraction method of microsporidium DNA based on a 3D printing functive, a reagent kit and an application of the extraction method, and belongs to the technical field of a nucleic acid extraction method and application. Samples are subjected to splitting treatment with lysate, DNA extraction is realized through holding a 3D printing special-shaped functive by hand or machine to quick transfer in lysate, cleaning liquid and eluent, time-consuming tedious steps of pipetting, centrifuging and the like are not needed, the manual labor density is low, the cost is low, theflux is high, DNA quality is high, requirements for equipment and operating space are small, and the extraction method has automated and high-flux downstream application prospects. The invention alsoprovides an application reagent kit of the nucleic acid extraction method, and an application of the nucleic acid extraction method to PCR rapid diagnosis for microsporidium pathogeny in animal and plant hosts. The problems that through a present microsporidium in vitro PCR diagnosing technique, target nucleic acid preparation is complex and time-consuming and the flux is low can be solved, the extraction method can be widely applied to fast detection of various microsporidium, and the detection flux, the operation convenience, the detection sensitivity and the detection accuracy are notablyincreased.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid extraction methods and applications, and in particular relates to a method for extracting microsporidian DNA based on 3D printing functional bodies, a kit and applications thereof. Background technique [0002] Microsporidia, a diverse phylum, can parasitize almost all types of animals, among which insects and fish are the main hosts. It has been reported that pathogenic microsporidia have potential risks to human health through the food chain, and their transmission among economic insects (silkworms and bees) and between fish will not only cause disease outbreaks but also reduce production , and also affect the food safety of downstream products (silkworm chrysalis, honey, fish meat) as food sources. [0003] Through the diagnosis technology of microsporidia, timely detection of microsporidia and cutting off its transmission are of great significance for the prevention and control and produ...

Claims

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Application Information

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IPC IPC(8): C12N15/10C12Q1/686C12Q1/04B08B3/08B33Y80/00
CPCC12N15/1006C12Q1/686B08B3/08B33Y80/00C12Q2531/113
Inventor 李佩佩姜晓滨李蒙航袁志杰叶博赵振军岳冬梅姜欣雨范琦张波王林美
Owner LIAONING OCEAN & FISHERIES SCI RES INST
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