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Group of SNP markers related to radioactive oral mucositis and application thereof

A technique for oral mucositis and radioactivity, applied in the field of SNP markers

Active Publication Date: 2020-09-15
SUN YAT SEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is to overcome the problems of radioactive oral mucositis caused by existing radiotherapy and the deficiencies of prevention and treatment measures. The nucleotide polymorphisms were typed, and the highly specific and sensitive SNPs that were highly correlated with radiotherapy-induced radiation oral mucositis were screened out, and a corresponding risk prediction diagnostic kit that was convenient for clinical application was developed. It can provide data support for the prevention and control of inflammation, and provide data support for the exploration and discovery of new drugs with potential therapeutic value; it can predict and intervene in advance the occurrence of oral mucositis in patients with nasopharyngeal carcinoma treated with radiotherapy, and improve the treatment efficiency and life of tumor patients quality

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  • Group of SNP markers related to radioactive oral mucositis and application thereof
  • Group of SNP markers related to radioactive oral mucositis and application thereof

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Experimental program
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Effect test

Embodiment 1

[0045] The collection of embodiment 1 sample and the collation of data

[0046] 1. Sample inclusion criteria:

[0047] (1) Nasopharyngeal carcinoma patients with clear pathological diagnosis and clinical stage;

[0048] (2) Functional status score (KPS) ≥ 70 points;

[0049] (3) Age 18-80 years old;

[0050] (4) Have complete medical records (medical history, physical examination, related examinations, previous treatments);

[0051] (5) Participate voluntarily and sign the informed consent form.

[0052] At the same time, patients with any of the following conditions were excluded:

[0053] (6) The patient has not completed all radiotherapy plans;

[0054] (7) Distant metastases have occurred when the patient is first diagnosed;

[0055] (8) The patient has received head and neck radiotherapy for other diseases (except nasopharyngeal carcinoma).

[0056] 2. The inventor collected a large number of blood samples from the Sun Yat-Sen University Cancer Hospital Biobank fro...

Embodiment 2

[0057] Whole Genome Scanning of Example 2 Sample Peripheral Blood DNA

[0058] We recorded whether radiation-induced oral mucositis occurred and the severity of radiation-induced oral mucositis among the 1467 patients with nasopharyngeal carcinoma who received radiation therapy and met the enrollment conditions. At the same time, Illumina Human610-Quad and Infinium Global Screening Array-24 bead genome-wide chip scans were performed on these 1467 patients with nasopharyngeal carcinoma, and genome-wide association analysis was performed.

[0059] Specifically, among the 1467 samples that met the above conditions, 349 patients with severe radiation oral mucositis (grade ≥ 3) and 1118 patients with mild radiation oral mucositis (grade ≤ 2) were obtained. The two groups of people were detected by Illumina Human610 Quad and Infinium Global Screening Array-24 bead chips to obtain relevant results.

[0060] 1. Use the phenol-chloroform method to extract peripheral blood genomic DNA,...

Embodiment 4

[0091] Example 4 Production of the auxiliary diagnostic kit related to radiation oral mucositis

[0092] In the genome-wide SNP scanning using the Illumina Human 610-Quad chip and the Infinium Global ScreeningArray-24 bead chip, the SNP sites significantly related to the occurrence of radiation-induced oral mucositis were determined, and the found SNP sites were further analyzed through the construction of statistical models. The combination of SNP sites was screened out as the detection sites of the kit. The kit includes specific primers and probes for these SNPs (see SEQ ID NO. 1-84 in the sequence listing). There can also be commonly used reagents required by corresponding PCR techniques, such as dNTPs, MgCl , double distilled water, fluorescent probes, etc. and blank control, etc.). The value of this kit is that it only needs peripheral blood and does not require other tissue samples. It uses specific primers and fluorescent probes to detect SNPs, and calculates the mode...

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Abstract

The invention discloses a group of SNP markers related to radioactive oral mucositis caused by radiotherapy and application thereof. The invention researches and screens an SNP marker combination related to the radioactive oral mucositis, and the combination can be used as a marker for evaluating the radioactive oral mucositis occurrence risk of a nasopharyngeal carcinoma patient. The invention also prepares a kit for clinical radioactive oral mucositis risk assessment or diagnosis. The risk of the radioactive oral mucositis caused by radiotherapy can be predicted more comprehensively and wellby combining the two factors of a radiotherapy technology and a treatment means. The kit has the advantages of being stable, minimally invasive, easy to detect, efficient and convenient. The markersare applied to clinic, protective measures are taken in advance for a high-risk patient suffering from the radioactive oral mucositis, individualized treatment of the patient is achieved, the long-term life quality of the patient is improved, and effective implementation of a radiotherapy scheme and the treatment effect of the patient are guaranteed.

Description

technical field [0001] The invention belongs to the technical field of oncology and molecular diagnosis. More specifically, it relates to a SNP marker associated with radiation-induced oral mucositis and its application. Background technique [0002] Radiotherapy is the preferred treatment for head and neck tumors such as nasopharyngeal carcinoma, but oral mucositis caused by radiotherapy is one of the most common acute adverse reactions. This adverse reaction often easily causes patients to have difficulty eating, weight loss, fatigue, pain, and decreased sleep quality, and even severe oral mucositis will lead to interruption of radiotherapy, thereby affecting the treatment process of cancer patients. Once radiation oral mucositis occurs, opioid analgesics are generally used clinically to relieve symptoms, but they cannot be effectively relieved for most patients. Therefore, it is particularly important to predict the risk of radiation-induced oral mucositis in patients w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G16H50/30
CPCC12Q1/6883C12Q2600/106C12Q2600/156G16H50/30
Inventor 贾卫华杨大伟王曈旻
Owner SUN YAT SEN UNIV