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Method for building protein/phospholipid/cholesterol multi-heterozygous micro-scale vesicles based on hydrophobic interaction

A hydrophobic interaction and protein technology, applied in the field of vesicle model preparation, can solve the problems of poor stability of phospholipid vesicles, high permeability of protein vesicles, and lack of fluidity of membranes.

Active Publication Date: 2020-09-18
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the shortcomings of existing single-component vesicles, such as poor stability of phospholipid vesicles, poor permeability of polymer vesicles, almost no fluidity in the membrane, and high permeability and poor fluidity of protein vesicles. problem, and provides a method for constructing protein / phospholipid / cholesterol multivariate hybrid micro-scale vesicles based on hydrophobic interactions

Method used

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  • Method for building protein/phospholipid/cholesterol multi-heterozygous micro-scale vesicles based on hydrophobic interaction
  • Method for building protein/phospholipid/cholesterol multi-heterozygous micro-scale vesicles based on hydrophobic interaction
  • Method for building protein/phospholipid/cholesterol multi-heterozygous micro-scale vesicles based on hydrophobic interaction

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specific Embodiment approach 1

[0024] Embodiment 1: A method for constructing protein / phospholipid / cholesterol multivariate hybrid micro-scale vesicles based on hydrophobic interactions in this embodiment is specifically carried out according to the following steps:

[0025] 1. Add BSA-NH 2 Dissolve in DMSO reagent, and then add sodium bicarbonate buffer solution dropwise to prepare BSA-NH 2 solution; the BSA-NH 2 The concentration of the solution is 1.0~2.0mg / mL; the BSA-NH 2 The volume ratio of the mass of the sodium bicarbonate buffer solution to the sodium bicarbonate buffer solution is 1mg:(0.1~0.5) mL; the concentration of the sodium bicarbonate buffer solution is 25~50mmol / L; DMSO and H in the DMSO reagent 2 The volume ratio of O is 1:(5~10);

[0026] Two, Chol-COOH is dissolved in the DMSO reagent, then dropwise adds sodium bicarbonate buffer solution wherein, is mixed with cholesterol solution; The concentration of described cholesterol solution is 0.02~0.1mg / mL; The quality of described Chol-CO...

specific Embodiment approach 2

[0033] Specific embodiment two: the difference between this embodiment and specific embodiment one is: BSA-NH described in step one 2 The concentration of the solution was 1.5 mg / mL. Other steps and parameters are the same as those in the first embodiment.

specific Embodiment approach 3

[0034] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the concentration of the cholesterol solution in step 2 is 0.04 mg / mL. Other steps and parameters are the same as those in Embodiment 1 or 2.

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Abstract

The invention belongs to the technical field of vesicle model preparation, and specifically relates to a method for building protein / phospholipid / cholesterol multi-heterozygous micro-scale vesicles based on hydrophobic interaction. In order to solve the deficiency of existing single component vesicles and the problems of poor phospholipid vesicle stability, poor polymer vesicle permeability, little fluidity of membranes, high protein vesicle permeability and poor fluidity, the method is provided and includes the following steps: 1, grafting Chol-COOH to the surface of BSA-NH2; 2, coupling theBSA-NH2 to PNIPAM to obtain BSA-Chol / PNIPAM; 3, building protein vesicles; and 4, assembling phospholipid and the protein vesicles into the multi-heterozygous micro-scale vesicles based on hydrophobicinteraction. The multi-heterozygous micro-scale vesicles are built by taking the main components of natural cell membranes as primitives.

Description

technical field [0001] The invention belongs to the technical field of vesicle model preparation, and in particular relates to a method for constructing protein / phospholipid / cholesterol multivariate hybrid microscale vesicles based on hydrophobic interactions. Background technique [0002] The cell membrane is mainly composed of phospholipids, proteins, glycolipids and cholesterol. The phospholipid bilayer constitutes the basic skeleton of the cell membrane. Proteins are embedded in the phospholipid bilayer to play a supporting role. The presence of cholesterol can effectively regulate the fluidity of phospholipids. , Various components have different functions and characteristics in the cell membrane, and it is this diversity of components and functions that provides the possibility for the normal operation of cells. Inspired by the cell structure in nature, the scientists constructed a vesicle model similar to the natural cell structure. A vesicle is an ordered structure ...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K47/42A61K47/24A61K47/28
CPCA61K9/1273A61K47/42A61K47/24A61K47/28
Inventor 刘小曼徐军林幼萍黄鑫
Owner HARBIN INST OF TECH
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