Screening method and application of SNP molecular marker associated with Nandan Yao egg shell color

Abstract

The invention discloses a screening method of an SNP molecular marker associated with Nandan Yao egg shell color. The screening method comprises the following steps: (1) finding out genes related to egg quality from known literatures, designing primers, taking Nandan Yao chicken, collecting blood, extracting genomic DNA, and performing sequencing in a mixed pool; (2) taking Nandan Yao hens as testchickens, measuring eggshell color and shape indexes of eggs from 24 weeks old and to 60 weeks old; (3) collecting blood of the 60-week-old test chicken, extracting genome DNA, and performing PCR sequencing on detected SNP sites; and (4) sorting sequencing data and eggshell color data, screening out candidate SNP loci significantly associated with eggshell colors, and providing an SNP molecular marker theoretical basis for eggshell color breeding. The method can be applied to rapid and simple cultivation for improving the eggshell color of Nandan Yao chicken eggs, simplifies whole genome association analysis, directly performs SNP marker screening on trait-related genes, and is suitable for breeding of quantitative traits with high heredity.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a screening method and application of Nandanyao egg shell color-associated SNP molecular markers. Background technique [0002] With the improvement of people's living standards, more and more attention has been paid to the appearance of eggs. Studies have shown that eggshell color is not directly related to egg quality inside eggs, but consumers often regard eggshell color as an important indicator of egg quality, and eggshell color has a greater impact on consumers' desire to buy. At the same time, the preference of eggshell color in different regions will directly affect the sales and selling price of eggs in the market. Consumers not only pay attention to the color of eggshells, but also pay attention to the uniformity of eggshell color. For example, some markets have a uniform dark brown color. Eggs are more expensive, and in some other markets light brown shell e...

AI Technical Summary

Problems solved by technology

At present, the molecular mechanism of the formation of green-shelled eggs has been clearly analyzed, while the formation of brown-shelled eggs includes a series of processes such as the synthesis, deposition and transport of protoporphyrin IX, which are jointly regulated by multiple genes, showing a series of color shades. variation, but there are few studies on the formation mechanism of the eggshell color of brown-shelled eggs

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