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Method for quickly identifying and evaluating cold resistance of cryptomeria fortunei

A technology of cold resistance and cedar, which is applied in the field of crop science and can solve problems such as high or low cold resistance

Active Publication Date: 2020-09-25
NANJING FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Patent application number 200910216387.3 bamboo cold resistance identification and evaluation method, 201610830631.5 a winter rapeseed cold resistance identification and evaluation method, 201711172417.6 coconut cold resistance identification method based on digital models, all proposed a model for evaluating cold resistance based only on plants, namely comprehensive evaluation method, but in practical application, the evaluation system involves many indicators, and it will inevitably be inconvenient to obtain the cold resistance of plants quickly and easily.

Method used

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  • Method for quickly identifying and evaluating cold resistance of cryptomeria fortunei
  • Method for quickly identifying and evaluating cold resistance of cryptomeria fortunei
  • Method for quickly identifying and evaluating cold resistance of cryptomeria fortunei

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Embodiment 1

[0028] Eleven cedar clones (#3, #25, #32, #57, #74) in Yangmeiling Forest Farm (118°31′54.56″E, 25°22′24.09″N) in good growth state without pests and diseases were selected. , #42, #54, #66, #68, #011, #X1). In June 2014, pruning shears were used to cut out branches that were semi-lignified, robust, free from diseases and insect pests, with short internodes and 2 to 3 lateral buds in the current year to make cuttings. When cutting, the upper incision of the cutting is cut flat, and the position is 1-1.5cm away from the upper bud; the lower incision is obliquely cut, about 45° inclined, and the inclined surface is opposite to the direction of the upper bud; the upper and lower cuts are required to be smooth, and prevent splitting, and keep the upper bud. , the cuttings are about 12-15cm long. After the cuttings are cut, keep them as moist as possible, and soak them in water in time. The soaking time is generally more than 12 hours. 0.1% carbendazim disinfection for 2h. Soak ...

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Abstract

The invention discloses a method for rapidly identifying and evaluating cold resistance of cryptomeria fortunei, and belongs to the technical field of crop science. The method mainly comprises the following steps: (1) carrying out cutting propagation of a clone of cryptomeria fortunei to be detected; (2) performing low-temperature treatment on the cryptomeria fortunei cutting seedlings; (3) measuring the change conditions of cell membrane permeability, semi-lethal temperature, malondialdehyde, SOD and soluble protein content of the low-temperature stressed cryptomeria fortunei leaves; (4) evaluating the cold resistance so as to determine the strength of the cold resistance of the cryptomeria fortunei to be detected; and (5) carrying out cold resistance verification and further screening. According to the method, on the basis that the cold resistance of the cryptomeria fortunei is comprehensively evaluated, it is found that the cold resistance of more than 80% of the clone of the cryptomeria fortunei can be quickly judged without damage by enabling clonal in-vitro lateral branches of cryptomeria fortunei to be subjected to low-temperature stress at -4 DEG C and measuring the relative conductivity, so that a method for rapidly identifying and evaluating the cold resistance of the cryptomeria fortunei is further provided, and the method has very important significance for reasonable planning and layout of utilization, planting and the like of cryptomeria fortunei germplasm resources and cold resistance breeding.

Description

technical field [0001] The invention belongs to the technical field of crop science and relates to a method for identification and evaluation of cold resistance of plants, in particular to a method suitable for rapid identification and evaluation of cold resistance of different cedar types. Background technique [0002] Low temperature is one of the important environmental factors affecting its growth, productivity and ecological distribution. Globally, hundreds of billions of dollars are lost each year due to reduced agricultural and forestry production due to low temperatures or cold damage. At present, there is no fundamental solution to low temperature damage. Therefore, it is of great significance to study the effect of low temperature on plant growth and improve the tolerance of plants to low temperature. [0003] Cedar is a unique tree species in China. It is produced in areas below 1100 meters above sea level in Tianmu Mountain in Zhejiang, 3800 Kan in Nanping, Fuj...

Claims

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Application Information

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IPC IPC(8): G01N27/02G01N33/00G01N1/42A01G17/00A01G7/06
CPCG01N27/02G01N33/0098G01N1/42A01G17/005A01G7/06
Inventor 徐进张莹婷张萌胡海亮
Owner NANJING FORESTRY UNIV
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