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Carbohydrate lactic acid bacteria metabolism analysis and detection device and method

A detection device and a technology for lactic acid bacteria, applied in the field of biosensors, can solve problems such as poor subjectivity and accuracy, and achieve the effects of reducing signal drift and reducing costs

Active Publication Date: 2020-10-02
天津海星辉科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to address the shortcomings of the prior art, to propose a carbohydrate lactic acid bacteria metabolic analysis detection device and method, to solve the problems of strong subjectivity and poor accuracy of conventional taste analysis and detection in the prior art

Method used

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  • Carbohydrate lactic acid bacteria metabolism analysis and detection device and method
  • Carbohydrate lactic acid bacteria metabolism analysis and detection device and method
  • Carbohydrate lactic acid bacteria metabolism analysis and detection device and method

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Embodiment 1

[0025] Such as figure 1 As shown, the carbohydrate lactic acid bacteria metabolism analysis and detection device 100 provided by the present invention includes: a controller 110, a signal circuit 120, a light source modulation circuit 130, an excitation light source 140, an optical addressable potential sensor 150, a reference electrode 160, and a test chamber 170. The test cavity 170 is fixed on the front of the optical addressable potential sensor 150 , and the reference electrode 160 is inserted into the top cover of the test cavity 170 to apply a bias voltage to the optical addressable potential sensor 150 . The optical addressable potential sensor 150 is packaged on the PCB through pads, and connected to the signal circuit 120 for signal collection. The PCB board packaged with the optical addressable potential sensor 150 is fixed to the bottom of the test cavity 170 for detection. The excitation light source 140 is arranged on the back of the optical addressable potentia...

Embodiment 2

[0042] The catabolism of sugar by lactic acid bacteria mainly includes two pathways, namely, the glycolysis pathway (homolactic fermentation) and the phosphoketolase pathway (heterolactic fermentation). The glycolytic pathway (embden-meverhef-parnus pathway, EMP) refers to the fermentation of glucose to lactic acid and adenosine triphosphate (ATP) under anaerobic conditions. The phosphoketolase pathway (PK) refers to the glycolysis of glucose to produce equal amounts of lactic acid, ethanol, and CO under anaerobic conditions. 2 and ATP. Therefore, lactic acid bacteria will produce lactic acid that affects the taste when metabolizing sugar. The sugar lactic acid bacteria metabolism analysis and detection device provided by the present invention is used to monitor the catabolism of lactic acid bacteria to different sugars by detecting changes in pH. Such as Figure 4 As shown, the carbohydrate lactic acid bacteria metabolic analysis detection method of the present invention i...

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Abstract

The invention relates to a carbohydrate lactic acid bacteria metabolism analysis and detection device which comprises a controller, a signal circuit, a light source modulation circuit, an excitation light source, a light addressing potential sensor, a reference electrode and a test cavity, the light addressing potential sensor is fixed at the bottom of the test cavity and is connected with the signal circuit; one end of the reference electrode is inserted into the test cavity, and the other end is connected with the signal circuit to provide bias for the light addressable potential sensor; theexcitation light source is arranged on the back surface of the light addressing potential sensor, is connected with the light source modulation circuit and provides light excitation for the light addressing potential sensor; the light source modulation circuit is connected with the signal circuit and is controlled by the signal circuit to modulate optical excitation; and the controller is in communication connection with the signal circuit and is used for detecting, controlling, recording and processing acquired data. According to the invention, quantitative analysis and detection of carbohydrate lactic acid bacteria metabolism can be realized.

Description

technical field [0001] The present application relates to the field of biosensors, in particular to a device and method for analyzing and detecting carbohydrate lactic acid bacteria metabolism. Background technique [0002] Taste is a sensation produced by food stimulating the chemical sensory system of taste organs in the human mouth, including four basic tastes: sweet, sour, bitter, and salty. The various tastes that people usually taste are the result of the mixture of these four tastes. The taste receptors are the taste buds on the tongue. The taste buds are oval in shape and mainly composed of taste cells and supporting cells. There are microvilli on the top of the taste cells extending toward the taste hole, which are in contact with saliva. The base of the cells is innervated by nerve fibers. Chemicals from food dissolve in saliva and come into contact with taste receptor cells through taste pores, where they interact with taste receptors or ion channels. These inte...

Claims

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Application Information

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IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 边仿
Owner 天津海星辉科技有限公司
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