Method for analyzing substituent-containing naphthalene ring
An analytical method and technology of substituents, applied in the field of pharmaceutical analysis, can solve the problems of lack of quality control standards for compounds, and achieve the effect of good separation effect, simple analysis method and good peak shape.
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Embodiment 1
[0032] In this embodiment, the following methods are used to analyze the naphthalene ring structure containing substituents:
[0033] (compound 1) and (compound 2).
[0034] Accurately weigh the appropriate amount of the above two compounds, put them in the same measuring bottle, add a mixed solvent of acetonitrile and water with a volume ratio of 50:50 to dissolve, and dilute to make about 0.5 mg of hydrochloric acid compound 2 and 0.5 mg of hydrochloric acid compound 1 in every 1 mL. , 5 μg and shake well.
[0035] The liquid chromatographic conditions of the analytical method are: the chromatographic column model is Chiralpak AY-RH, 150×4.6mm, 5 μm, the detection wavelength is 222 nm, the column temperature is 25°C, the flow rate is 0.4 mL / min, and the injection volume is 10 μL , the mobile phase is a mixed solution of ammonium bicarbonate aqueous solution and acetonitrile with a concentration of 20mmol / L, and the volume ratio is 50:50. The results are as follows figu...
Embodiment 2
[0039] The difference between this example and Example 1 is that the flow rate of this example is 0.5 mL / min, and the rest are analyzed the same as Example 1. The results showed that the resolution of compound 1 and compound 2 was 9.45.
Embodiment 3
[0041] The difference between this embodiment and Example 1 is that the concentration of the ammonium bicarbonate aqueous solution is 25 mmol / L, and all the others are analyzed the same as in Example 1. The results showed that the resolution of compound 1 and compound 2 was 9.67.
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