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Method for simultaneously determining DON toxin and NIV toxin

A toxin and retention time technology, applied in the field of detection, can solve the problems of complex experimental process, low efficiency of DON toxin and NIV toxin detection, inaccurate measurement results, etc., to achieve accurate results, excellent recovery rate and linear relationship, and convenient operation Effect

Inactive Publication Date: 2020-10-09
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is mainly to solve the problems of inefficient detection of DON toxin and NIV toxin in the measurement of wheat and other grains, inaccurate measurement results, complicated experimental process, etc., and to provide a new, convenient and accurate result for simultaneous determination of DON toxin and NIV way of toxin

Method used

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  • Method for simultaneously determining DON toxin and NIV toxin
  • Method for simultaneously determining DON toxin and NIV toxin
  • Method for simultaneously determining DON toxin and NIV toxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Materials and Methods

[0045] 1.1 Main instruments and reagents

[0046] Table 1. Main instruments and reagents

[0047]

[0048]

[0049] 1.2 Standards and reagents

[0050] Table 2. Standards and reagents

[0051]

[0052] experimental method

[0053] 2.1 Pretreatment of test products

[0054] Accurately weigh 25g (accurate to 0.01g) of the sample and place it in a 250ml Erlenmeyer flask and add 100ml of acetonitrile:water solution (84 / 16, v / v). Then ultrasonically extract at 35°C for 30 min, and filter with quantitative filter paper after standing still.

[0055]Take 10ml of the above filtrate and transfer to Mycosep#227 or Mycosep#226 (romer) multifunctional purification column for processing. Take 4 ml of the treated effluent, place it in a glass tube, and dry it with a nitrogen blower at 55°C. Then take out the glass tube, add 1ml of mobile phase (water: acetonitrile: methanol = 90:5:5, v / v / v) to dissolve, and then filter through a 0.22um organic ...

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Abstract

The invention discloses a method for simultaneously determining DON toxin and NIV toxin in cereals. According to the method, the DON toxin and the NIV toxin are simultaneously detected by using the liquid chromatograph after the sample is purified by using the multifunctional purification column so that the experimental process is greatly shortened, and the recovery rate and the precision are relatively high. The method is simple, effective, economical and reliable, and provides a new technical guarantee for the safety of grain products.

Description

technical field [0001] The invention belongs to the detection field and relates to a method for measuring mycotoxins in grains, in particular to a method for simultaneously measuring DON toxin and NIV toxin. Background technique [0002] Scab, also known as wheat "cancer", is more serious in wheat-growing areas in central my country, and its pathogenesis is complex. It is generally caused by Fusarium fungi, which infect wheat when it is heading and flowering. The metabolic toxins of Gibberella mainly include DON toxin and its derivatives, NIV toxin, ZEN toxin, FB toxin, etc. These toxins more or less affect the health and safety of humans and livestock. [0003] Deoxynivalenol (DON toxin), also known as vomitoxin, is because people or livestock will produce a series of symptoms such as diarrhea, dizziness, vomiting, abdominal pain, nausea after exposure to a certain amount of the toxin, and it has certain carcinogenicity. One of the common mycotoxins in cereal pollutants in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8675G01N2030/062
Inventor 陈璨彭逸卢杰梁坤司红起马传喜
Owner ANHUI AGRICULTURAL UNIVERSITY