Sheet-like cell-cultured product for regeneration of gastrointestinal tract
A technology of sheet cells and cultures, applied in animal cells, tissue regeneration, vertebrate cells, etc., can solve problems such as perforation, and achieve the effects of promoting healing, preventing complications, and improving prognosis
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
example 1
[0191] Example 1. Fabrication of Sheet Cell Culture
[0192] (1) Sheet-shaped porcine skeletal muscle myoblast culture
[0193] The striated muscle of the pig's lower limbs was collected under general anesthesia, and the collected tissue was treated with an enzymatic digestion solution containing collagenase and trypsin to disperse into single cells. The above cells were incubated at 37°C, 5% CO 2 The cells were cultured in MCDB131 medium containing 15% FBS to confluence under the condition of , and the cells were recovered. The recovered cells were divided into 2.2×10 7 A 60mm temperature-responsive Petri dish (UpCell (R) , Cell Seed) were cultured in DMEM / F12 medium containing 20% FBS for 12 hours. The temperature was then lowered to 20°C, whereby the sheet-like cell culture was peeled off from the dish and recovered. A reinforcement layer was formed from fibrin gel on the above sheet cell culture.
[0194] The sheet-shaped porcine skeletal muscle myoblast cultures...
example 2
[0197] Example 2. Production of Duodenal ESD Pig Model
[0198] Duodenal ESD was performed under general anesthesia on pigs from which striated muscle was collected in Example 1 (1), and they were used as a slice treatment group (n=5). In addition, duodenal ESD was also performed on control pigs under general anesthesia as a control group (n=7). That is, as duodenal ESD, a part of the lumen surface of the porcine duodenum was peeled off. On the serosal surface of the outer side of the duodenum corresponding to the ESD operation site (dissection site of the lumen surface of the duodenum) of pigs in the slice treatment group, so as to completely cover the dissection site of the lumen surface Paste (apply) the sheet-shaped skeletal muscle myoblast culture made in Example 1 (1). Furthermore, the pedicled omentum sheet was applied from above (outside) the adhered sheet-shaped skeletal muscle myoblast culture so as to completely cover the dissected part of the lumen surface. In...
PUM
| Property | Measurement | Unit |
|---|---|---|
| Diameter | aaaaa | aaaaa |
| Diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More