Preparation method of cyclic gamma-polyglutamic acid modified hydrogel loaded with growth factors
A technology of growth factor and polyglutamic acid, which is applied in the field of biotechnology and pharmacy, can solve the problems that have not been reported, and achieve the effects of promoting cell proliferation, good biocompatibility, and promoting local wound healing
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Embodiment 1
[0041] Embodiment 1: the preparation of cyclo-γ-PGA modified PNIPAM hydrogel
[0042] cyclo-γ-PGA ( figure 1 ) Modified PNIPAM hydrogel: Mix the two substances according to PNIPAM / cyclo-γ-PGA in order of 100:0, 75:25, 50:50, and 25:75, and the total concentration of the solution is 20% (w / v) , fully mixed at room temperature for 3 hours, overnight at 4°C to remove air bubbles, and gelled in a water bath at 37°C. It can be observed that the dehydration of PNIPAM hydrogels has been effectively improved when PNIPAM / cyclo-γ-PGA is 75:25 or 50:50 ( figure 2 ). Under the scanning electron microscope, it was observed that when the ratio of PNIPAM / cyclo-γ-PGA was 75:25, the hydrogel showed a typical porous structure ( image 3 ).
Embodiment 2
[0043] Embodiment 2: the preparation of the cyclo-γ-PGA modified PNIPAM hydrogel of loading EGF
[0044] Mix PNIPAM with a final concentration of 150 mg / mL and cyclo-γ-PGA 50 mg / mL, mix thoroughly at room temperature for 3 hours, and place in an environment at 4°C. Fully dissolve EGF into a 1 mg / mL solution with an appropriate amount of deionized water, mix according to the ratio of hydrogel solution / EGF solution of 100:1, place in a refrigerator at 4°C, and shake on a shaker for 30 seconds every 3 minutes. The time was 30 min, and after mixing, a 10 μg / mL cyclo-γ-PGA-modified PNIPAM hydrogel solution loaded with growth factors was obtained. Finally, the homogeneously mixed solution was placed in a water bath at 37° C. to become a gel.
Embodiment 3
[0045] Example 3: Preparation of cyclo-γ-PGA modified PNIPAM hydrogel loaded with bFGF
[0046]Mix PNIPAM with a final concentration of 150 mg / mL and cyclo-γ-PGA 50 mg / mL, mix thoroughly at room temperature for 3 hours, and place in an environment at 4°C. Fully dissolve bFGF into a 1 mg / mL solution with an appropriate amount of deionized water, mix according to the ratio of hydrogel solution / bFGF solution of 100:1, place in a refrigerator at 4°C, and shake on a shaker for 30 seconds every 3 minutes. The time was 30 min, and after mixing, a 10 μg / mL cyclo-γ-PGA-modified PNIPAM hydrogel solution loaded with growth factors was obtained. Finally, the homogeneously mixed solution was placed in a water bath at 37° C. to become a gel.
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