Nanovesicles derived from enhydrobacter bacteria, and use thereof
A technology of vesicles and water bacteria, which is applied in the fields of bacterial medical raw materials, applications, and functions of food ingredients, can solve the problems of unreported vesicle secretion, no diagnosis and treatment, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0072] Example 1. Analysis of In vivo Absorption, Distribution and Excretion Patterns of Gut Bacteria and Bacteria-Derived Vesicles
[0073] To assess whether gut bacteria and bacterial-derived vesicles are absorbed systemically through the gastrointestinal tract, the following experiments were performed. A dose of 50 μg of each of the fluorescently labeled enterobacteria and the vesicles derived from the enterobacteria was administered to the stomach of the mouse via the gastrointestinal tract, and at 0 minutes, 5 minutes, 3 hours, 6 hours and Fluorescence was measured after 12 hours. As a result of observing the whole image of the mouse, as Figure 1A As shown, bacteria were not taken up systemically, but vesicles derived from bacteria were absorbed systemically at 5 minutes after administration, and strong fluorescence was observed in the bladder at 3 hours after administration, making it possible to see that the vesicles were excreted. to the urinary tract. Furthermore...
Embodiment 2
[0075] Example 2. Metagenomic analysis of bacterial-derived vesicles in clinical samples
[0076] After first placing the blood sample into a 10 ml tube and allowing the suspension to settle by centrifugation (3,500 xg, 10 min, 4°C), only the supernatant was transferred to a new 10 ml tube. After removing bacteria and impurities using a 0.22-μm filter, transfer them to a Centriprep tube (centrifugal filter 50 kD) and centrifuge at 1,500 × g and 4 °C for 15 min, discard the material smaller than 50 kD, and The product was concentrated to 10ml. After bacteria and impurities were removed again using a 0.22-μm filter, supernatant was discarded and aggregated pellets were dissolved by performing ultracentrifugation at 150,000×g and 4°C for 3 hours using a 90Ti type rotor in normal saline (PBS).
[0077] 100 μl of vesicles isolated by the method described above were boiled at 100 °C to extract internal DNA from lipids, and then cooled on ice for 5 min. Then, in order to remove ...
Embodiment 3
[0081] Example 3. Metagenomic Analysis of Bacteria-Derived Vesicles in the Blood of Pancreatic Cancer Patients
[0082] Genes were extracted from the vesicles present in the blood of 176 pancreatic cancer patients and 271 normal individuals (both groups were matched in age and sex). The distribution of vesicles from bacteria of the genus Aquatobacter was assessed. As a result, it was confirmed that vesicles derived from bacteria of the genus Aquatus significantly decreased in blood from pancreatic cancer patients compared with blood from normal individuals (see Table 2 and Table 2). figure 2 ).
[0083] [Table 2]
[0084]
PUM
| Property | Measurement | Unit |
|---|---|---|
| diameter | aaaaa | aaaaa |
| absorbance | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More