A method for identifying animal origin of livestock and poultry meat
A technology of animal origin and livestock and poultry meat, which is applied in the field of identification of animal origin of livestock and poultry meat, and achieves the effect of fast speed, high practical value, and easy mastery
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Embodiment 1
[0053] Sample pretreatment method
[0054] 1. Fresh samples
[0055] Use commercially available qualified chicken, duck, goose, pig, beef, mutton, etc. as materials, and fully stir until it is like minced meat.
[0056] 2. Basic samples of absolute dry matter
[0057] (1) Use commercially available fresh chicken, duck, goose, pig, beef, mutton, etc. as ingredients, and stir well until it becomes minced meat;
[0058] (2) Dry in an oven at 45°C for 5-6 hours to make an air-dried sample, grind it into powder, and wait for hydrolysis to determine the amino acid content;
[0059] (3) At the same time, take an appropriate amount of powdery air-dried samples and place them in an oven at 105°C±1°C for another 4 hours to calculate the dry matter rate;
[0060] (4) Convert the amino acid content of the air-dried sample to the amino acid content of the absolute dry substance basic sample.
[0061] 3. Sample hydrolysis
[0062] (1) Weigh the sample, weigh 50mg for fresh sample, weig...
Embodiment 2
[0073] Establishment of Amino Acid Patterns in Fresh Muscle Samples of 6 Animals
[0074] 1. Determination of 16 kinds of amino acids in 6 kinds of animals
[0075] The contents of 16 kinds of amino acids are quite different among different animals, and the relative contents and coefficient of variation of the 16 kinds of amino acids are shown in Table 1.
[0076] Table 1 Contents and variation coefficients of 16 kinds of amino acids in muscles of 6 kinds of animals
[0077] Unit: mg / 100g
[0078]
[0079]
[0080] Continued Table 1
[0081]
[0082] 2. Principal component analysis
[0083] Correlation analysis showed that most of the amino acids were moderately or above correlated, indicating that there was information overlap in the selected initial variables, and principal component analysis could be performed. Principal component analysis was performed on 16 kinds of amino acids, and the characteristic rate and contribution rate of each principal component wer...
Embodiment 3
[0103] Establishment of amino acid patterns of 6 kinds of basic samples of animal muscle absolute dry matter
[0104] 1. Determination of 16 kinds of amino acids in 6 kinds of animal muscle absolute dry matter basic samples
[0105] The determination results of 16 kinds of amino acids in the basic samples of muscle dry matter of 6 kinds of animals showed that the contents of 16 kinds of amino acids were quite different among different animals. See Table 7 for details.
[0106] Table 7 Contents and variation coefficients of 16 kinds of amino acids in 6 kinds of animal muscle absolute dry matter basic samples
[0107] Unit: mg / 100g
[0108]
[0109] Continued Table 7
[0110]
[0111]
[0112] 2. Principal component analysis
[0113] Correlation analysis showed that most of the 16 kinds of amino acids were correlated with each other more than moderately, indicating that the selected initial variables had overlapping information, and principal component analysis coul...
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