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Peucedanum praeruptorum rooting culture medium

A technology of rooting medium and basic medium, applied in the field of rooting medium of Peucedanum, can solve problems such as low rooting rate of Peucedanum test-tube seedlings, waste of resources, and affecting the efficiency of tissue culture and rapid propagation of Peucedanum, and achieve improvement of rooting efficiency, rooting The effect of increasing the rate and improving the survival rate of transplanting

Active Publication Date: 2020-10-30
ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Tissue culture is one of the means of artificial rapid propagation of Peucedanum. However, the rooting rate of Peucedanum tube seedlings in the existing tissue culture technology is not high, only about 40%, which affects the tissue culture and rapid propagation efficiency of Peucedanum. Cause test tube seedlings to brown and die, resulting in waste of resources

Method used

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  • Peucedanum praeruptorum rooting culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Prepare rooting medium of Peucedanum, the specific components are as follows:

[0016] Every 1000 mL of said rooting medium contains α-naphthalene acetic acid 0.1 mg, indole acetic acid 0.1 mg, neodymium chloride 0.10 mM, rooting powder 0.2 mg, agar powder 3 g, sucrose 28 g, and the balance is 1 / 2 MS medium.

Embodiment 2

[0018] Prepare rooting medium of Peucedanum, the specific components are as follows:

[0019] The rooting medium per 1000 mL contains α-naphthalene acetic acid 0.3 mg, indole acetic acid 0.3 mg, neodymium chloride 0.18 mM, rooting powder 0.8 mg, agar powder 5 g, sucrose 34 g, and the balance is 1 / 2 MS medium.

Embodiment 3

[0021] Prepare rooting medium of Peucedanum, the specific components are as follows:

[0022] The rooting medium per 1000 mL contains 0.15 mg of α-naphthalene acetic acid, 0.15 mg of indole acetic acid, 0.14 mM of neodymium chloride, 0.4 mg of rooting powder, 4 g of agar powder, 31 g of sucrose, and the balance is 1 / 2 MS medium.

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Abstract

The invention discloses a Peucedanum praeruptorum rooting culture medium. Every 1000mL of the Peucedanum praeruptorum rooting culture medium contains 0.1-0.3mg of alpha-naphthylacetic acid, 0.1-0.3mgof indoleacetic acid, 0.10-0.18mM of water-soluble neodymium-containing compounds, 0.2-0.8mg of rooting powder, 3-5g of agar powder, 28-34g of cane sugar and the balance of a basic culture medium. Through the rooting culture medium disclosed by the invention, the rooting rate of Peucedanum praeruptorum test-tube plantlets can be increased to 90% or above, and besides, the single-plant rooting number can also be increased to 5 or above, so that the transplanting survival rate of the Peucedanum praeruptorum test-tube plantlets can be greatly increased.

Description

technical field [0001] The invention relates to the technical field of Peucedanum tissue culture, in particular to a medium for rooting Peucedanum. Background technique [0002] Peucedanum is a perennial herb of Umbelliferae, its roots are irregularly cylindrical, conical or spindle-shaped, and the surface is dark brown or grayish yellow. Peucedanum is a commonly used Chinese herbal medicine. The dried root is used as medicine. It has the effect of lowering qi and resolving phlegm, dispelling wind and clearing heat. [0003] Tissue culture is one of the means of artificial rapid propagation of Peucedanum. However, the rooting rate of Peucedanum tube seedlings in the existing tissue culture technology is not high, only about 40%, which affects the tissue culture and rapid propagation efficiency of Peucedanum. Cause test-tube seedlings to brown and die, resulting in waste of resources. Contents of the invention [0004] Based on the technical problems existing in the backg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 黄和平黄鹏汪电雷彭华胜
Owner ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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