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Novel DNA hydrogel and preparation method thereof

A new type of hydrogel technology, which is applied in the fields of pharmaceutical formulations, medical preparations of non-active ingredients, medical science, etc., can solve the problem of high production cost of DNA hydrogel, and achieve low cost, simple operation process and simple process Effect

Active Publication Date: 2020-10-30
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] The purpose of the present invention is to overcome the problem of high production cost of DNA hydrogel in the prior art and provide a novel DNA hydrogel

Method used

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  • Novel DNA hydrogel and preparation method thereof
  • Novel DNA hydrogel and preparation method thereof
  • Novel DNA hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A kind of preparation method of novel DNA hydrogel, with plasmid pRset-eGFP (purchased in: Promega Corporation) as template as example, comprises the following steps:

[0044] The specific sequence of the primer strand DNA with methacrylamide modification is shown in Table 1.

[0045] Table 1. DNA sequences

[0046]

[0047] (1) Add primer chain DNA with methacrylamide modification at a final concentration of 0.4 μmol / L, 2×PfuPCR MasterMix, 15ng of plasmid DNA, and the balance is ultrapure water, and perform PCR reaction to obtain methacrylamide base long-chain DNA (1235bp), see figure 1 ;

[0048] Place the mixed liquid in a PCR instrument for the following temperature control procedures:

[0049] 94℃3min

[0050] 94℃30s

[0051] 55℃30s

[0052] Return to the second step at 72°C for 2 minutes and continue for 29 cycles

[0053] 72℃10min

[0054] 4℃Pause

[0055] (2) The long-chain DNA (1235bp) modified by methacrylamide group was concentrated to 5 μmol / L, a...

Embodiment 2

[0058] A kind of preparation method of novel DNA hydrogel, with plasmid pRset-eGFP (purchased in: Promega Corporation) as template as example, comprises the following steps:

[0059] The specific sequence of the primer strand DNA with methacrylamide modification is shown in Table 2.

[0060] Table 2. DNA sequences

[0061]

[0062] (1) Add primer chain DNA with methacrylamide modification at a final concentration of 0.4 μmol / L, 2×PfuPCR MasterMix, 15ng of plasmid DNA, and the balance is ultrapure water, and perform PCR reaction to obtain methacrylamide Base long-chain DNA (1235bp);

[0063] Place the mixed liquid in a PCR instrument for the following temperature control procedures:

[0064] 94℃3min

[0065] 94℃30s

[0066] 55℃30s

[0067] Return to the second step at 72°C for 2 minutes and continue for 29 cycles

[0068] 72℃10min

[0069] 4℃Pause

[0070] (2) The long-chain DNA (1235bp) modified by methacrylamide group was concentrated to 5 μmol / L, and the 2 Under ...

Embodiment 3

[0072] A kind of preparation method of novel DNA hydrogel, with plasmid pRset-eGFP (purchased in: Promega Corporation) as template as example, comprises the following steps:

[0073] The specific sequence of the primer strand DNA with methacrylamide modification is shown in Table 3.

[0074] Table 3. DNA sequences

[0075]

[0076]

[0077] (1) Add primer strand DNA with methacrylamide modification at a final concentration of 0.4 μmol / L, 2×PfuPCR MasterMix, 15ng of plasmid DNA, and the balance is ultrapure water, and perform PCR reaction to obtain methacrylamide Long DNA (1235bp);

[0078] Place the mixed liquid in a PCR instrument for the following temperature control procedures:

[0079] 94℃3min

[0080] 94℃30s

[0081] 55℃30s

[0082] Return to the second step at 72°C for 2 minutes and continue for 29 cycles

[0083] 72℃10min

[0084] 4℃Pause

[0085] (2) The long-chain DNA (1235bp) modified by methacrylamide group was concentrated to 6 μmol / L, and the 2 Unde...

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Abstract

The invention discloses novel DNA hydrogel and a preparation method thereof. The preparation method comprises the steps that 1, plasmid DNA serves as a template, and methacrylamide long-chain DNA is obtained through a PCR technology under the action of methacrylamide modified primer chains; and (2) carrying out free radical polymerization on the methacrylamide long-chain DNA under the action of aninitiator and a catalyst to obtain the DNA hydrogel. According to the DNA hydrogel prepared by the method, an amido bond is used as a cross-linking point, the gelling mode is simple, the long-chain DNA molecules are obtained through the PCR technology, the synthesis cost of the DNA hydrogel is reduced, the guidance effect is realized on the construction of a DNA hydrogel system, and the DNA hydrogel can be used in the fields of acellular protein synthesis, medical dressings, immunotherapy, drug sustained release and the like.

Description

technical field [0001] The invention relates to a novel DNA hydrogel and a preparation method thereof. Background technique [0002] Deoxyribonucleic acid (DNA), as a biological macromolecule that encodes, stores and transmits genetic information, is one of the core molecules of a living system [1] . In 1982, Seeman [2] For the first time, the cross-shaped structure was designed and synthesized by using the base complementarity of DNA, realizing the design and precise assembly of nanoscale static structures, and opening the door to DNA nanotechnology. From the point of view of materials and chemistry, DNA sequences can be arranged in any combination according to design requirements, and there are a series of mature molecular biology methods to provide rich tools for molecular manipulation of DNA, such as polymerase chain reaction (PCR), In addition, there are many methods for chemical modification of DNA, based on which DNA has designability, diversity and multifunctional...

Claims

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Application Information

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IPC IPC(8): C08J3/075A61L26/00A61K47/26C08L99/00
CPCC08J3/075A61L26/008A61L26/0061A61L26/0009A61K47/26C08J2399/00A61L2400/06C08L99/00
Inventor 李凤仰大勇余文婷
Owner TIANJIN UNIV
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