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A method for improving the efficiency of root-knot nematode resistance of oily radish

A technology against root-knot nematodes and oil radish, applied in nematicides, botanical equipment and methods, chemicals for biological control, etc., can solve the problem of ineffective anti-root-knot nematodes and toxicity to humans and ecological hazards , the active ingredients of oily radish are not high, and achieve the effects of diverse transmission routes, wide host range, and increased porosity

Active Publication Date: 2022-01-14
云南省农业科学院生物技术与种质资源研究所
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0005] Although oily radish carries resistance genes against cyst nematodes and root-knot nematodes, the current application of oily radish to root-knot nematodes is mainly to ferment oily radish straw with other raw materials and strains to prepare fertilizers. Because oily radish is effective The ingredients are not high, so the effect against root-knot nematodes is not obvious
For decades, the control of root-knot nematode disease has mainly relied on chemical pesticides, but long-term use has caused root-knot nematode resistance, and its toxicity has caused harm to humans and ecology and has been banned. Therefore, the development of a new type of pesticide can effectively prevent It is of great significance to control root-knot nematode disease and reduce the use of chemical fertilizers

Method used

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  • A method for improving the efficiency of root-knot nematode resistance of oily radish
  • A method for improving the efficiency of root-knot nematode resistance of oily radish
  • A method for improving the efficiency of root-knot nematode resistance of oily radish

Examples

Experimental program
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Effect test

Embodiment 1

[0020] A method for improving the anti-root-knot nematode efficiency of oily radish, specifically comprising the following steps:

[0021] (1) Preparation of oily radish straw powder: pick the oily radish stems and leaves after the oily radish is mature, dry the oily radish stems in an oven at 65°C for 20 hours until the water content is 13%, then crush them with a pulverizer and pass through 200 mesh Sieve the resulting powder for later use.

[0022] (2) The oily radish straw powder was added into distilled water at a ratio of 100 g / L, soaked for 45 hours, centrifuged at 12000 rpm for 10 minutes, and the supernatant was collected.

[0023] (3) Concentrate the supernatant of step (2) until the solid content accounts for 55% to obtain the initial extract; add fulvic acid to the initial extract at a ratio of 120g / L to obtain the final root-knot nematode inhibitor .

Embodiment 2

[0025] A method for improving the anti-root-knot nematode efficiency of oily radish, specifically comprising the following steps:

[0026] (1) Preparation of oily radish straw powder: pick the oily radish stems and leaves after the oily radish is mature, dry the oily radish stems and leaves under natural conditions for 15%, then crush them with a pulverizer and pass through a 150-mesh sieve, and the resulting powder is ready for use .

[0027] (2) The oily radish straw powder was added into distilled water at a ratio of 110 g / L, soaked for 55 hours, centrifuged at 12000 rpm for 10 minutes, and the supernatant was collected.

[0028] (3) Concentrating the supernatant of step (2) until the solid content accounts for 60% to obtain the initial extract; adding fulvic acid to the initial extract at a ratio of 110g / L to obtain the final root-knot nematode inhibitor .

Embodiment 3

[0030] A method for improving the anti-root-knot nematode efficiency of oily radish, specifically comprising the following steps:

[0031] (1) Preparation of oily radish straw powder: pick the oily radish stems and leaves after the oily radish is mature, dry the oily radish stems and leaves under natural conditions until the water content is 12%, then crush them with a pulverizer and pass through a 300-mesh sieve , and the resulting powder is ready for use.

[0032] (2) Add oily radish straw powder into distilled water at a ratio of 90 g / L, soak for 50 h, centrifuge at 12000 rpm for 10 min, and collect the supernatant.

[0033] (3) Concentrating the supernatant of step (2) to a solid content ratio of 56% to obtain the initial extract; adding fulvic acid to the initial extract at a ratio of 100g / L to obtain the final root-knot nematode inhibitor .

[0034] Get the extract in Examples 1 to 3 of the present invention, and study its inhibitory effect on root-knot nematodes. The ...

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Abstract

The invention discloses a method for improving the anti-root-knot nematode efficiency of oily radish, which specifically comprises the following steps: picking the stems and leaves of oily radish after the oily radish is mature, crushing the oily radish straw with a pulverizer after drying, passing through a 150-300 mesh sieve, The obtained powder is ready for use; add the oily radish straw powder into distilled water at a ratio of 90-110g / L, soak for 45-55 hours, and collect the supernatant after centrifugation; concentrate the supernatant to obtain the initial extract; press 100-120g Fulvic acid was added to the primary extract at a ratio of / L. The method of the invention has simple process and strong practicability, and can obtain a relatively high flavonoid-rich solution by extracting the effective components of the radish stalks with water, and can improve the anti-root-knot nematode efficiency of the radish.

Description

technical field [0001] The invention relates to a method for improving the root-knot nematode resistance efficiency of radish, belonging to the technical field of liquid fertilizer preparation. Background technique [0002] Root-knot nematode is considered the most invasive plant parasitic nematode. It has strong adaptability, diverse transmission routes, and a wide range of hosts. It can parasitize more than 3,000 kinds of plants. It is the most harmful to Solanaceae, Cucurbitaceae, and Cruciferous plants. agricultural production; according to incomplete statistics, the global annual crop loss caused by root-knot nematode damage is as high as 100 billion US dollars. [0003] The most common root-knot nematode pathogens that infect crops are Meloidogyneincognita, M.arenaria, M.hapla, and M.javanica4 kind. They are all distributed worldwide, and account for more than 90% of the crop losses caused by root-knot nematodes. In particular, root-knot nematodes have a large area ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/00A01N65/08A01N61/00A01P21/00A01P5/00
CPCA01N65/00A01N65/08A01N61/00
Inventor 余萍张绍松罗红梅李树红赵永昌董超
Owner 云南省农业科学院生物技术与种质资源研究所
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