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Multistage enzymolysis method of animal protein and application

An animal protein and protease technology, applied in the field of proteolysis, can solve the problems of difficult to achieve uniform and stable molecular weight distribution, limit the purification and extraction of small molecule products, and limit the development of small molecule proteins, and achieve high synchronous rate of enzymatic digestion points and high yield. The effect of improving the efficiency and wide application space

Pending Publication Date: 2020-11-03
王新丽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Taking collagen production as an example, it is difficult to achieve a uniform and stable molecular weight distribution in conventional large-scale production, which limits the purification and extraction of small molecule products, thus limiting the development of the small molecule protein market. Many studies cannot obtain a large number of accurate molecular weights. However, it is impossible to achieve industrial transformation and technology research and development

Method used

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  • Multistage enzymolysis method of animal protein and application
  • Multistage enzymolysis method of animal protein and application
  • Multistage enzymolysis method of animal protein and application

Examples

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Embodiment 1

[0050] Raw material: 100kg of fish intestine offal;

[0051] Pretreatment: Physical shredding, crushing and colloid milling of animal source raw materials to pulp;

[0052] Primary hydrolysis: Add 1 times the mass of water to the slurry, stir evenly, and heat the material to 75°C for 3 hours to hydrolyze at a constant temperature;

[0053] Primary biochemical degradation: add appropriate amount of cold water, stir evenly to keep the slurry at 50°C, adjust the pH value to 9.0, add alkaline protease with a mass of 0.04% of the raw material, stir evenly, and react at constant temperature for 40 minutes;

[0054] Secondary biochemical degradation: adjust the pH value of the slurry to 7.5, the temperature is 53°C, add alkaline protease with a mass of 0.05% of the raw material, stir evenly, and react at a constant temperature for 30 minutes;

[0055] Three-level biochemical degradation: adjust the pH value of the slurry to 6.8, and the temperature is 50°C, add papain with a mass of...

Embodiment 2

[0063] Raw materials: minced chicken (including skin and cartilage) 100kg;

[0064] Pretreatment: Physical shredding, crushing and colloid milling of animal source raw materials to pulp;

[0065] Primary hydrolysis: Add 1 times the mass of water to the slurry, stir evenly, and heat the material to 75°C for 3 hours to hydrolyze at a constant temperature;

[0066] Primary biochemical degradation: Add appropriate amount of cold water, stir evenly to keep the slurry at 53°C, adjust the pH value to 9.0, add alkaline protease with a mass of 0.04% of the raw material, stir evenly, and react at constant temperature for 50 minutes;

[0067] Secondary biochemical degradation: adjust the pH value of the slurry to 7.5, the temperature is 53°C, add alkaline protease with a mass of 0.05% of the raw material, stir evenly, and react at a constant temperature for 40 minutes;

[0068] Three-stage biochemical degradation: adjust the pH value of the slurry to 7.0, and the temperature is 50°C, ad...

Embodiment 3

[0076] Raw materials: small miscellaneous fish and minced fish 100kg;

[0077] Pretreatment: Physical shredding, crushing and colloid milling of animal source raw materials to pulp;

[0078] Primary hydrolysis: Add 1 times the mass of water to the slurry, stir evenly, and heat the material to 75°C for 3 hours to hydrolyze at a constant temperature;

[0079] Primary biochemical degradation: add appropriate amount of cold water, stir evenly to keep the slurry at 54°C, adjust the pH value to 8.5, add alkaline protease with a mass of 0.03% of the raw material, stir evenly, and react at constant temperature for 30 minutes;

[0080] Secondary biochemical degradation: adjust the pH value of the slurry to 7.5, the temperature is 52°C, add alkaline protease with a mass of 0.04% of the raw material, stir evenly, and react at a constant temperature for 25 minutes;

[0081] Three-stage biochemical degradation: adjust the pH value of the slurry to 7.0, and the temperature is 50°C, add pap...

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Abstract

The invention relates to a multistage enzymolysis method of an animal protein and an application, and belongs to the technical field of proteolysis. The multistage enzymolysis method of the animal protein comprises the following steps: pretreating an animal-derived raw material; mixing slurry with water, and performing and hydrolyzing; and sequentially performing first-stage biochemical degradation, second-stage biochemical degradation, third-stage biochemical degradation, fourth-stage biochemical degradation and fifth-stage biochemical degradation on the primary hydrolysis slurry, and performing filtering to obtain the protein subjected to multi-stage enzymolysis. Compared with an existing animal protein enzymolysis method, the method disclosed by the invention has the advantages of beingstable in enzymolysis effect, high in molecular weight uniformity, high in target molecule yield, low in relative cost and the like.

Description

technical field [0001] The invention relates to the technical field of proteolysis, in particular to a multi-stage enzymolysis method and application of animal protein. Background technique [0002] With the development and wide application of biotechnology, the technology of extracting specific natural substances from natural animals and plants has become an important means of obtaining natural nutritional raw materials. Plant extract technology mostly adopts subcritical, supercritical, alcohol or ether extraction schemes, which can Efficient separation of specific characteristic molecules. In the field of animal extraction, enzymatic hydrolysis technology and fermentation technology are mainly used as the main means. General enzymatic hydrolysis technology focuses on activity and average analysis volume, regardless of molecular weight distribution. [0003] Taking collagen production as an example, it is difficult to achieve a uniform and stable molecular weight distribut...

Claims

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Application Information

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IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 王新丽
Owner 王新丽