Online production method for bottle-cultivated pleurotus eryngii liquid culture

Abstract

The invention discloses an online production method for a bottle-cultivated pleurotus eryngii liquid culture. The online production method comprises the following steps of: (1) checking an inoculationchamber and equipment; (2) cleaning a fermentation tank and detecting leakage; (3) adding water for carrying out volume determination and regulating a pH value; (4) preparing a culture medium; (5) connecting with a steam discharging pipe: installing a T-branch pipe on the fermentation tank, connecting the steam discharging pipe to one branch of the T-branch pipe through an air feeding soft pipe,and independently connecting the other branch of the T-branch pipe to an inoculation machine and the fermentation tank through an inoculation pipe; (6) sterilizing and cooling culture materials; (7) carrying out steam disinfection and cooling on an inoculation spray head and the inoculation pipe; (8) carrying out transportation and inoculation; (9) carrying out cultivation and detection; and (10)finishing inoculation. According to the online production method, an amount of purification workshop personnel can be reduced, a pollution risk is lowered, inoculation efficiency is improved, an areaof a seed manufacturing zone is reduced, a seed manufacturing and utilizing flow is simplified, culture production cost is effectively lowered, and manufacture and utilization of the liquid culture can be more intensively, reasonably, effectively and accurately managed and controlled.

Description

technical field [0001] The invention relates to the technical field of production of bottle-grown Pleurotus eryngii liquid strains, in particular to an on-line production method of bottle-grown Pleurotus eryngii liquid strains. Background technique [0002] Strains are the basis and key to the development of the edible fungi industry. There are two main types of strains of edible fungi, namely solid strains and liquid strains. Sawdust species are the main species; with the great development of edible fungi, especially Pleurotus eryngii, the main shortcomings of solid strains such as long cultivation period, many intermediate links, heavy labor, high production cost, and low inoculation efficiency are more and more exposed. The more fully; the lack of solid strains has promoted the application and development of liquid species of Pleurotus eryngii in production, but the production of liquid species is currently the off-line production model of liquid species of Pleurotus eryn...

AI Technical Summary

Problems solved by technology

[0002] Strains are the basis and key to the development of the edible fungi industry. There are two main types of strains of edible fungi, namely solid strains and liquid strains. Sawdust species are the main species; with the great development of edible fungi, especially Pleurotus eryngii, the main shortcomings of solid strains such as long cultivation period, many intermediate links, heavy labor, high production cost, and low inoculation efficiency are more and more exposed. The more fully; the lack of solid strains has promoted the application and development of liquid species of Pleurotus eryngii in production, but the production of liquid species is currently the off-line production model of liquid species of Pleurotus eryngii in Japan and South Korea; but this liquid strain The disadvantage of this offline production mode is that the fermenter needs to occupy an autoclave separately, the fermenter takes up a lot of space, and ingredients, sterilization, inoculation and cleaning can not be completed in one place. Compared with the existing solid strain production, Although the production efficiency has been improved, the production cost has also been reduced, but the production efficiency still needs to be further improved, and the production cost also needs to be further reduced; due to the limitation of the sterilizing pot and the sterilization method of the off-line sterilized fermenter, each fermenter The maximum volume can only reach 600L, and the maximum daily output can only reach 60,000 bottles, which cannot meet the needs of the current production scale and the production of more liquid strains per unit area, as well as the needs of intensive production of cultivars

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