Method for constructing sesame recombinant inbred line
A construction method and technology of inbred lines, applied in the fields of molecular biology and genetic breeding, can solve problems such as partial segregation of population traits, and achieve the effects of reducing partial segregation, saving manpower input, and saving costs
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[0027] Example: Construction of Sesame Plant Height Recombinant Inbred Line (RIL) Population
[0028] (1) In the summer of 2013, at the Wuchang Experimental Base of the Oil Crop Research Institute of the Chinese Academy of Agricultural Sciences, the high-stalk sesame material AG150 (plant height 1.8m) was selected as the male parent and the short-stalk material AG151 (plant height 80cm) was selected as the female parent. Separate planting, select two male parent (AG150①, AG150②) and female parent (AG151①, AG152②) during the flowering period for plant-to-plant hybridization. After normal maturity, the hybrid seeds of male parent, female parent and F0 generation are harvested, and the listing mark is ZW105① and ZW105②.
[0029] (2) In the winter of 2013, the F0 generation hybrid seeds harvested above were used to plant F1 generations ZW105① and ZW105②, male parents AG150① and AG150②, female parents AG151① and AG152② plants in Sanya, Hainan, using conventional thinning and fixed seedl...
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