Method for constructing sesame recombinant inbred line

A construction method and technology of inbred lines, applied in the fields of molecular biology and genetic breeding, can solve problems such as partial segregation of population traits, and achieve the effects of reducing partial segregation, saving manpower input, and saving costs

Active Publication Date: 2020-11-10
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0007] One of the technical problems to be solved by the present invention is to provide a method for constructing a recombinant inbred line of sesame, which can effectively solve the problem of partial separation of p

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  • Method for constructing sesame recombinant inbred line

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Example Embodiment

[0027] Example: Construction of Sesame Plant Height Recombinant Inbred Line (RIL) Population

[0028] (1) In the summer of 2013, at the Wuchang Experimental Base of the Oil Crop Research Institute of the Chinese Academy of Agricultural Sciences, the high-stalk sesame material AG150 (plant height 1.8m) was selected as the male parent and the short-stalk material AG151 (plant height 80cm) was selected as the female parent. Separate planting, select two male parent (AG150①, AG150②) and female parent (AG151①, AG152②) during the flowering period for plant-to-plant hybridization. After normal maturity, the hybrid seeds of male parent, female parent and F0 generation are harvested, and the listing mark is ZW105① and ZW105②.

[0029] (2) In the winter of 2013, the F0 generation hybrid seeds harvested above were used to plant F1 generations ZW105① and ZW105②, male parents AG150① and AG150②, female parents AG151① and AG152② plants in Sanya, Hainan, using conventional thinning and fixed seedl...

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Abstract

The invention relates to a method for constructing a sesame recombinant inbred line. According to the method for constructing the sesame recombinant inbred line, a conventional method is broken through, single-plant harvesting and strain propagation methods are adopted all the time from the F3 generation to the F8 generation, the same part of equivalent capsules are picked, mixed harvesting and mixed planting are performed, namely single-plant planting is used for replacing plant rows, and the planting area is reduced by 80% or above; in the F3 generation to the F7 generation, through sparse sowing planting, thinning and final singling are avoided, partial separation caused by manual selection is reduced, and manpower investment is saved; in the harvesting stage, traditional single-plant selection harvesting, single-plant numbering and single-plant storage are replaced by picking the capsules at the same parts of single plants, so that the manpower input is reduced, and meanwhile, partial separation caused by manual selection during harvesting is reduced; and the method is simple, convenient, feasible, labor-saving, less in land occupation and less in required equipment, and the problem of population segregation caused by thinning and final singling can be well solved.

Description

technical field [0001] The invention belongs to the technical field of molecular biology and genetic breeding, and in particular relates to a method for constructing a recombinant inbred line of sesame. Background technique [0002] Sesamum (Sesamum indicum L.) is one of the seven major oil crops in the world, and it is also a traditional characteristic oil crop in my country. It is planted in various places. my country is the main producer of sesame, and its total output is second only to India. Sesame seeds are rich in unsaturated fatty acids, vitamins and trace elements such as calcium, iron, zinc, etc., have a good taste, fragrance and quality, are beneficial to human health, and are deeply loved by people. With the improvement of living standards, my country's sesame consumption continues to grow rapidly, from 590,000 tons in 2003 to 1.64 million tons in 2017, an increase of nearly two times, accounting for nearly 30% of the world's total consumption, and my country has...

Claims

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 王林海盛晨许方涛周瑢黎冬华张艳欣张秀荣
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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