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Application of propofol to improvement of cognitive function of mice by promoting formation of brain-developing dopamine neural network

A brain dopamine and neural network technology, applied in the field of biomedicine, can solve the problems of unknown research methods on the pharmacological effects of anesthetic drugs, and the mechanism of action needs to be explored, and achieve the effect of great clinical significance and social value.

Inactive Publication Date: 2020-11-13
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Due to the particularity of the developmental period, the unknown pharmacological effects of anesthetic drugs, and the limitations of research methods, the effects of the anesthetic drug propofol on infants and young children are still inconclusive, and in-depth research is still needed to explore, and the corresponding mechanism of action still needs to be explored

Method used

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  • Application of propofol to improvement of cognitive function of mice by promoting formation of brain-developing dopamine neural network
  • Application of propofol to improvement of cognitive function of mice by promoting formation of brain-developing dopamine neural network
  • Application of propofol to improvement of cognitive function of mice by promoting formation of brain-developing dopamine neural network

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Effects of Propofol on Learning and Memory Abilities and Emotional Responses of Neonatal Suckling Mice as Adults.

[0021] Experimental method: Newborn 7-day-old C57BL / 6 suckling mice were randomly assigned to blank control group, solvent control group and propofol group. The solvent control group was intraperitoneally injected with 50 μl of 10% fat emulsion (Intralipid), and the propofol group was intraperitoneally injected with 50 μl of 2-6 mg / kg propofol (Diprivan), respectively marked and recorded, and the growth of the mice was observed. The Morris water maze test and tail suspension test were performed on the mice at 2 months of age.

[0022] Experimental results: In the Morris water maze, the descending trend of the escape latency and the average swimming distance of the mice in the propofol group for the first three days of training was significantly different from that of the blank control group and the solvent control group (such as figure 1 A, 1B)...

Embodiment 2

[0023] Example 2: The effect of propofol on the proliferation and differentiation of neural stem cells in the hippocampal DG region of neonatal rats.

[0024] Experimental method: Newborn 7-day-old C57BL / 6 suckling mice were randomly assigned to blank control group, solvent control group and propofol group. -6mg / kg propofol (Diprivan) 50μl, respectively marked, recorded, and observed the growth of the mice. Six consecutive intraperitoneal injections of EdU were given within 3 days after drug exposure to label and trace proliferating cells. Observe the following at the respective time points:

[0025] Proliferation level of neural stem cells: After 3 days of drug exposure, brain tissue was perfused into mice, frozen sections were made, and Nestin immunofluorescent staining was performed on the sections. In the DG region of the hippocampus, count EdU + / Nestin + / DAPI + The average number of cells and the total number of cells (the total number of nuclei) indicate the proli...

Embodiment 3

[0028] Example 3: The effect of propofol on the formation of dopamine neural network in neonatal rats.

[0029] Experimental method: Newborn 7-day-old C57BL / 6 suckling mice were randomly assigned to blank control group, solvent control group and propofol group. -6mg / kg propofol (Diprivan) 50μl, respectively marked, recorded, and observed the growth of the mice. Six consecutive intraperitoneal injections of EdU were given within 3 days after drug exposure to label and trace proliferating cells. Observe the following:

[0030] The level of dopaminergic neuron generation: After 28 days of drug exposure, the brain tissue of the mouse was perfused and frozen, and the section was stained with TH, and the TH was counted in the VTA area of ​​the midbrain of the mouse + / EDU + / DAPI + The average number of cells and the total number of cells (the total number of nuclei) indicate the occurrence level of dopamine neurons.

[0031] Projection level of dopamine neurons in hippocampus CA...

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Abstract

The invention discloses application of propofol to improvement of a cognitive function of a mouse by promoting formation of a brain-developing dopamine neural network. The application has the beneficial effects that the influence of the propofol effect on the formation of the neural network of the suckling mouse and the change of long-term neural functions (including learning memory and emotionalanxiety) are deeply tracked through technologies such as cell tracing and omnibearing behavioral experiments, so that the application has important clinical significance and social value.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the use of propofol to improve the cognitive function of mice by promoting the formation of dopamine neural networks in the developing brain. Background technique [0002] 1. The neurotoxic / protective effect of general anesthetic drugs on the developing brain is controversial [0003] The effects of general anesthetics on the development of the nervous system and learning and memory ability of infants and young children have always been a controversial scientific research hotspot. The results of a large number of animal experiments and in vitro experiments have confirmed that general anesthetic drugs under experimental conditions, including NMDA antagonists and GABA agonists, will produce early neuroanatomical and related functional effects. In terms of clinical research, most trials have not found direct evidence that anesthesia drugs cause neurological damage in children...

Claims

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Application Information

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IPC IPC(8): A61K31/05A61P25/28
CPCA61K31/05A61P25/28
Inventor 冯霞姜美花陆迪涵陈柯宇
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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