Unlock instant, AI-driven research and patent intelligence for your innovation.

Induction method for efficiently producing nibea albiflora triploid all-female fishes

A technology for croaker and triploid, which is applied in fish farming, application, climate change adaptation, etc., can solve the problems of crocodile gynogenetic diploid growth speed and poor fish meat quality, so as to improve the development The effect of status, novel method and high safety

Inactive Publication Date: 2020-11-17
MARINE FISHERIES RES INST OF ZHEJIANG
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Its shortcoming is that the growth rate and fish meat quality of croaker gynogenetic diploid are not good enough

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Induction method for efficiently producing nibea albiflora triploid all-female fishes
  • Induction method for efficiently producing nibea albiflora triploid all-female fishes
  • Induction method for efficiently producing nibea albiflora triploid all-female fishes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] An induction method for efficiently producing triploid all-female croaker, comprising the steps of:

[0033] (1) Cultivate gynogenetic croaker and pseudo male croaker;

[0034] Specific method for obtaining gynogenetic yellow croaker: Dilute the semen of the stone bream with sperm diluent and place it at 1350 μW / cm 2 Inactivate under ultraviolet light, then mix the UV irradiated semen with mature yellow croaker eggs at a volume ratio of 1:11, add seawater to mix and inseminate for 2.5min, and immerse the fertilized eggs in seawater at 3.5°C Perform cold shock treatment for 13 minutes, and then incubate in seawater at 19°C for 36 hours until the membranes emerge to obtain gynogenetic croup fry;

[0035] The specific method of cultivating pseudo-realgar croaker: prepare feed containing letrozole, dissolve letrozole in 95% alcohol, then pour the solution into the feed, and prepare a compound feed containing letrozole 11mg / kg, and identify The gynogenetic yellow croaker f...

Embodiment 2

[0045] An induction method for efficiently producing triploid all-female croaker, comprising the steps of:

[0046] (1) Cultivate gynogenetic croaker and pseudo male croaker;

[0047] Specific method for obtaining gynogenetic yellow croaker: Dilute the semen of the stone bream with sperm diluent, and place it at 1300 μW / cm 2 Inactivated under ultraviolet light, then the UV-irradiated inactivated scorpion semen was mixed with mature yellow meager eggs at a volume ratio of 1:10, added to seawater and mixed for insemination for 2 minutes, and the fertilized eggs were immersed in seawater at 3°C. Cold shock treatment for 10 minutes, and then incubate in seawater at 18°C ​​for 34 hours until filming, to obtain gynogenetic yellow croup fry;

[0048] The specific method of cultivating pseudo-realgar croaker: prepare feed containing letrozole, dissolve letrozole in 94% alcohol, then pour the solution into the feed, and prepare a compound feed containing letrozole 10mg / kg, and identif...

Embodiment 3

[0058] An induction method for efficiently producing triploid all-female croaker, comprising the steps of:

[0059] (1) Cultivate gynogenetic croaker and pseudo male croaker;

[0060] Specific method for obtaining gynogenetic yellow croaker: Dilute the semen of the stone bream with sperm diluent and place it at 1400 μW / cm 2 Inactivate under ultraviolet light, then mix the UV-irradiated inactivated semen and mature yellow croaker eggs at a volume ratio of 1:12, add seawater to mix and inseminate for 3 minutes, and immerse the fertilized eggs in seawater at 4 °C Cold shock treatment for 15 minutes, and then incubate in seawater at 20°C for 38 hours until filming, to obtain gynogenetic yellow croup fry;

[0061] The specific method of cultivating pseudo-realgar croaker: prepare feed containing letrozole, dissolve letrozole in 96% alcohol, then pour the solution into the feed, and prepare a compound feed containing letrozole 12mg / kg. The gynogenetic yellow croaker fry started to...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a polyploid breeding method, and discloses an induction method for efficiently producing nibea albiflora triploid all-female fishes. The method aims at solving the problem ofblank induction technology of nibea albiflora triploid all-female fishes in the prior art, and comprises the following steps of (1) culturing gynogenesis nibea albiflora and pseudo-male nibea albiflora; (2) collecting pseudo-male nibea albiflora sperms and gynogenesis female fish eggs for performing artificial insemination on; and (3) performing triploid all-female fish fry induction and samplingdetection. The induction method is safe, efficient, novel, easy to operate and higher in practicability. The method opens up a new technical way for sex control breeding and polyploid breeding of sciaenidae fishes such as nibea albiflora. The established induction method for the nibea albiflora triploid all-female fishes can be used for efficiently inducing and producing nibea albiflora triploid all-female fish fries in batches. The induction method is novel, easy to operate, higher in practicability and high in safety.

Description

technical field [0001] The invention relates to a polyploid breeding method, in particular to an induction method for efficiently producing triploid all-female croakers. Background technique [0002] In aquaculture, because fish triploids have obvious advantages in terms of growth and meat yield compared with diploids, breeding triploids has become one of the effective methods for fish breeding. In addition, some fishes are sexually dimorphic, which shows that the female / male fish grows faster and weighs more than the other sex. Artificially induced triploid has been widely used in the research and practice of various fish genetic breeding at home and abroad, and has become an effective means of fish species improvement. Triploid fish can increase the growth rate of fish and improve the quality of fish meat, etc. It has shown great application value in aquaculture, and the research on triploid fish breeding has been favored by many researchers at home and abroad. Some spec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 汪波徐冬冬陈睿毅竺奇慧谭朋王立改
Owner MARINE FISHERIES RES INST OF ZHEJIANG