Breeding method of Wumai germplasm with different genetic background using blue dwarf wheat and purple wheat

A technology of blue dwarf and purple wheat, applied in the field of wheat breeding, to achieve the effect of high health and nutritional value

Active Publication Date: 2022-07-26
淄博爱民种业有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Up to now, no one has used blue dwarf blight to breed black wheat containing blue and purple genes, and no one has used black grains containing blue and purple genes as male parents to pollinate blue dwarf blight. Breeding has different genetics. Types of Umai

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Using Lanaibaishiluan 02-1 and Shannong Zimai No. 1 as materials to select and breed Wumai germplasm, including HF-Am1 Wumai strain and HF-Am2 Wumai strain, the specific process is as follows:

[0026] Breeding of Lanaibaishan Nongzimai No. 1: The blue grains (Lanaibai) sorted from the backcross population of Lanaibaishi Luan 02-1 were planted in autumn, planted in 2 rows, 2 meters long, 25 grains / m, row spacing 20 cm, sow 1 row on one side to pollinate the male parent Shannong Zimai No. 1, the distance between the male parent and the blue dwarf defeat is 30 cm, 30 grains / m, and the other side is sown before wintering. No. 1, 100 grains / meter, the planting belt covers an area of ​​0.8 meters, and a small walkway of 1 meter is left around the planting belt for experimental observation. Before heading, use bamboo stakes, iron wire, and plastic woven cloth to build a separation wall to build a separation area. Timely pull out all kinds of fertile plants with anthers tha...

Embodiment 2

[0035] Using Lanaibaiheima 1 and Luozhen 1 purple wheat as materials to select and breed Wumai germplasm-HF-Am3 and HF-Am4 Wumai lines.

[0036] The same approach and method as in Example 1 were adopted, using our self-bred Lanai Baiheima No. 1 and Luozhen No. 1 purple wheat to be transformed into Lanai Bailuozhen No. 1, and then in Lanai Bailuozhen No. 1 Breeding fertile plants with black grains, and then irradiated the selected black grains and the blue grains sorted by Lanai Bailuozhen No. 1, and arranged corresponding breeding procedures according to the characteristics of the mother's inheritance of the purple trait. Whole-grain plants were selected and bred, and the number of chromosomes was detected through line selection testing and root tip staining and microscopic examination to confirm the genetic type of the selected Wumai germplasm. Finally, a 4D / 4E translocation diploid Wumai line HF-Am3 with 44 chromosomes and a 4D / 4E translocation substitution line Wumai line H...

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Abstract

The invention belongs to the technical field of wheat breeding, and in particular relates to a method for selecting and breeding black wheat germplasm with different genetic backgrounds by using blue dwarf and purple wheat. A method for the aleurone layer blue gene of black wheat germplasm. Through continuous back-pollination of blue dwarf wheat with purple wheat, it is transformed into blue dwarf and purple wheat, and then the fertile black grain containing blue and purple genes isolated by breaking the linkage is selected from the transformed blue dwarf and purple wheat. Plant material, radiation-treated black grains and blue dwarf blue dwarf purple wheat, and then used the treated black grains to pollinate the treated blue dwarf blue dwarf blue wheat, and then select the homozygous whole black wheat line, and through root cap tissue compression, staining microscope Check the genetic background type of blue wheat germplasm that was bred. The method of the invention is reliable and efficient for breeding black wheat.

Description

technical field [0001] The invention belongs to the technical field of wheat breeding, in particular to a method for selecting and breeding black wheat germplasm with different genetic backgrounds by using blue dwarf wheat and purple wheat. Background technique [0002] Wheat is the main ration crop of the Chinese people, and it is also the most important staple food for the people in northern my country. The staple food can solve the "hidden hunger" caused by nutrient imbalance and relieve health risks. Yes, it is the same way to solve the problem of insufficiency and imbalance of nutrients such as trace elements and vitamins through biofortification. [0003] Blue wheat contains anthocyanins with antioxidant function in its aleurone layer and endosperm, and can enrich trace elements such as zinc, iron, chromium, manganese, selenium and other beneficial trace elements from the soil, which have strong health care effects. At the same time, the ground flour also has a good ta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/08
CPCA01H1/02A01H1/08
Inventor 耿爱民钟文李瑞杰于卫国耿冬梅耿一凡耿少楠耿名男
Owner 淄博爱民种业有限公司
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