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Photobioreactor for microalgae culture

A technology of photobioreactor and bioreactor, which is applied in the direction of photobioreactor, specific-purpose bioreactor/fermenter, combination of bioreactor/fermenter, etc. Problems such as uneven light reception by cells and low light energy utilization efficiency can achieve the effect of speeding up the shuttle cycle, improving the light energy utilization rate, and facilitating the subsequent process

Pending Publication Date: 2020-11-24
TSINGHUA UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The purpose of the embodiments of the present invention is to provide a photobioreactor for cultivating microalgae to solve the general problem of existing photobioreactors: with the increase of algae cell density, the light attenuation is enhanced, and the light path is shortened to cause the reaction The algae cells in different areas in the reactor receive uneven light. When the volume of the reactor becomes larger, the volume ratio of the light-dark area decreases rapidly, the light energy utilization efficiency is low, and the growth of algae cells is limited.

Method used

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  • Photobioreactor for microalgae culture
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  • Photobioreactor for microalgae culture

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Experimental program
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Effect test

Embodiment 1

[0053] The photobioreactor body 1 is made of high light-transmitting plexiglass, with a thickness of 5mm, an inner diameter of 180mm, and a reactor height of 500mm; the guide tube is made of plexiglass, with a thickness of 5mm, an inner diameter of 120mm, and a height of 360mm. The distance is 20mm; it is fixed on the bottom surface by a support rod; the diameter of the ring-shaped light source is 220mm, and there are 3 ring-shaped light sources distributed equidistantly on the upper, middle, lower, and each light-emitting ring is 700mm long. Cloth 35 LED patches with double core 3528 type and maximum power of 0.15W. It is actually measured that the light energy emitted by each rod-shaped light source is 10.5W, and the total light energy entering the culture solution is 31.5W. The light intensity on the outer surface of the reactor is 400μE m -2 the s -1 . The shell of the gas distributor at the bottom is made of stainless steel, and the ventilation surface is a stainless s...

Embodiment 2

[0055] Embodiment two (volume enlargement)

[0056] The photobioreactor body 1 is made of high light-transmitting plexiglass, with a thickness of 8mm, an inner diameter of 180mm, and a reactor height of 1.5m; the guide tube is made of plexiglass, with a thickness of 5mm, an inner diameter of 120mm, and a height of 1.35m. The distance from the bottom is 20mm; it is fixed on the bottom surface by a support rod; the diameter of the ring-shaped light source is 220mm, and 7 ring-shaped light sources are distributed equidistantly from top to bottom. 35 double-core 3528-type light-emitting diode patches with a maximum power of 0.15W are arranged in a circle. It is actually measured that the light energy emitted by each rod-shaped light source is 10.5W, and the total light energy entering the culture solution is 73.5W. The average light intensity on the outer surface of the reactor is 400μE m -2 the s -1 . The shell of the gas distributor at the bottom is made of stainless steel, ...

Embodiment 3

[0058] Embodiment three (ventilated stirring type)

[0059] The photobioreactor body 1 is made of high light-transmitting plexiglass, with a thickness of 5mm, an inner diameter of 180mm, and a reactor height of 500mm; there is no guide tube inside. The diameter of the circular ring-shaped light source is 220mm, and three ring-shaped light sources are distributed equidistantly from top to bottom. 35 LED patches for W. It is actually measured that the light energy emitted by each rod-shaped light source is 10.5W, and the total light energy entering the culture solution is 31.5W. The light intensity on the outer surface of the reactor is 400μE m -2 the s -1 . The shell of the gas distributor at the bottom is made of stainless steel, and the ventilation surface is a stainless steel sintered porous plate with a diameter of 40mm and an average pore size of 60μm. The total volume of the reactor is 12L, and the effective volume (liquid loading) is 10L. The stirring motor is fixe...

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Abstract

The invention discloses a photobioreactor for microalgae culture. The photobioreactor comprises a photobioreactor body, a ventilation and gas-liquid mixing device arranged at the bottom of the photobioreactor body, a flow guide device which is suspended in the photobioreactor body, divides the interior of the photobioreactor body into a light area and a dark area and is used for promoting liquid circulation and gas-liquid mass transfer, a light source arranged on the periphery of the photobioreactor body, and a stirring and axial flow propelling device which comprises an upper stirring paddleand a lower stirring paddle, wherein the lower stirring paddle is used for providing vortex to realize gas-liquid exchange, carbon dioxide mass transfer and oxygen desorption, and the upper stirring paddle is used for promoting shuttle circulation of algae liquid between the light area and the dark area. The photobioreactor is simple in structure and convenient to operate, the utilization efficiency of light energy can be remarkably improved for culturing different types of microalgae, and the photobioreactor can be widely applied to large-scale amplification culture of the microalgae.

Description

technical field [0001] The invention relates to the field of microalgae cultivation, in particular to a photobioreactor for microalgae cultivation. Background technique [0002] Microalgae are rich in protein, unsaturated fatty acids, algal polysaccharides, β-carotene, various trace elements (such as Cu, Fe, Se, Mn, Zn, etc.), vitamins and other high-value nutrients. and other fields are widely used. At present, it is also playing an increasingly important role in emerging fields such as renewable energy and environmental governance. [0003] The basis of the application of microalgae biotechnology lies in the low cost and large-scale cultivation of microalgae, and the core of the large-scale cultivation of microalgae is the photobioreactor. At present, there are mainly two types of open culture devices and closed photobioreactors. Open culture is the oldest and most widely used method. At present, countries all over the world, especially my country, still use it as the ma...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/02C12M1/04
CPCC12M21/02C12M27/08C12M29/06C12M27/18
Inventor 张荣庆章真
Owner TSINGHUA UNIV