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Lactobacillus mucosa of human origin and use thereof

A mucosal lactobacillus, a technology for use, applied in the field of microbiology, can solve problems such as side effects, hyperlipidemia cannot be properly controlled, local or systemic drug allergy or immune resistance, etc.

Active Publication Date: 2022-06-28
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some drugs are currently used to treat obesity and obesity-related diseases, these drugs often have some side effects
Hyperlipidemia in some patients is not adequately controlled by diet and / or exercise therapy or by the use of therapeutic compounds
Local or systemic allergy or immune resistance to the drug may also occur with the use of the drug
[0004] At present, there is no effective method and medicine for the treatment and prevention of obesity and its related diseases with little side effects

Method used

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  • Lactobacillus mucosa of human origin and use thereof
  • Lactobacillus mucosa of human origin and use thereof
  • Lactobacillus mucosa of human origin and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0130] Example 1 Isolation and identification of Lactobacillus mucosae A1 strain

[0131] 1.1 Isolation of human Lactobacillus mucosae A1 strain

[0132] Cut 0.6 g of GD02 children's fecal samples after 105 days of nutritional intervention, add 30 mL of Ringer working solution (0.1% L-Cysteine) in an anaerobic incubator, and vortex to mix well. Centrifuge at 200 g for 5 min, and take 1 mL of supernatant for serial dilution with Ringer (0.1% L-Cysteine) working solution. Drain 10 -1 -10 -5 200 μL of the dilution solution of these 5 dilution gradients was spread on MRS agar plates, and each gradient was spread on 3 pieces repeatedly, and incubated upside down at 37° C. under anaerobic conditions for 18 hours. The plates with more and clear colonies were selected, and 200 single colonies grown on the plates were randomly selected and streaked and purified on MRS agar plates twice.

[0133]The purified single bacterial isolates were respectively inoculated into MRS broth mediu...

Embodiment 2

[0157] Example 2 Growth curve of Lactobacillus mucosaeA1 strain

[0158] In this example, the growth curve of the Lactobacillus mucosae A1 strain in MRS medium was measured.

[0159] The specific method is as follows: streak the Lactobacillus mucosae A1 strain frozen at -80°C on the MRS plate, pick a single clone and culture it in 5mL MRS liquid medium overnight, and transfer it into 1% of the inoculum (v / v). In 150ml MRS liquid medium; after mixing, divide into 10 15mL EP tubes, each tube of 14ml bacterial liquid, take out a tube about every 2 hours before the plateau phase is observed to measure OD 600 value, then remove a tube about every 8 hours to measure OD 600 value. The cultivation process was carried out in an anaerobic environment, and three replicates were set for each growth curve measurement time point, and the measurement results were expressed as the mean ± standard error.

[0160] The results of the growth curve assay are as image 3 As shown, the growth of...

Embodiment 3

[0161] Example 3 Effect of Lactobacillus mucosaeA1 strain on ApoE in Western dietary environment - / - Alleviating effect of abnormal lipid metabolism in mice

[0162] 3.0 Experimental Materials:

[0163] Mice: This example uses ApoE - / - The genetic background of C57BL / 6 male mice was completed within the SPF barrier of the Experimental Animal Center of Shanghai Jiao Tong University. ApoE (apolipoprotein E) is one of the important apolipoproteins in plasma and is involved in lipid transport in blood.

[0164] Strain: Lactobacillus mucosae A1 strain isolated as described in Example 1

[0165] High-fat feed: Western diet high in fat and cholesterol, containing 21% fat and 0.15% cholesterol, purchased from Shanghai Fanbo Biotechnology Co., Ltd.

[0166] Normal maintenance feed: containing 4.8% fat, purchased from Jiangsu Synergy Medicine Bioengineering Co., Ltd.

[0167] 3.1 Experimental method:

[0168] 24 8-10 week old ApoE - / - Male mice were randomly divided into 3 groups...

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Abstract

The invention provides human-sourced mucosal lactobacilli and uses thereof. Specifically, the present invention provides a human-derived Lactobacillus mucosa. The present invention also provides compositions for treating and preventing obesity and related diseases, and methods for reducing body weight and / or blood fat. Experiments show that the mucosal lactobacillus of the present invention can reduce blood fat and atherosclerosis, so it can be used in the treatment and prevention of obesity and obesity-related diseases (such as cardiovascular diseases).

Description

technical field [0001] The present invention relates to the field of microorganisms. In particular, the present invention relates to human Lactobacillus mucosae and uses thereof, including in the treatment and prevention of obesity and related diseases. Background technique [0002] Obesity is a systemic chronic metabolic disease, usually accompanied by abnormal lipid metabolism, imbalance of glucose homeostasis and chronic inflammation. Intestinal flora is closely related to the occurrence of obesity, and imbalanced intestinal flora is an important factor inducing obesity. With the development of omics technology, those gut microbes associated with the development of obesity have been continuously discovered and become potential effective targets for obesity treatment. [0003] Drug therapy is currently the main clinical method for the treatment of obesity and obesity-related diseases (such as diabetes). Mechanisms of drug therapy include appetite suppression, increased ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P3/04A61P3/06A61P9/00A61P3/10A23L33/135C12R1/225
CPCA61K35/747A61P3/04A61P3/06A61P9/00A61P3/10A23L33/135A23V2002/00C12R2001/225C12N1/205A23V2400/11A23V2200/326A23V2200/3262A23V2200/328A23V2200/332
Inventor 张晨虹姜天翌赵立平
Owner SHANGHAI JIAOTONG UNIV
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