Wheat powdery mildew resistance related protein pm24 and its coding gene and application
A powdery mildew and wheat technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as impact, lack of new genes for resistance to powdery mildew, and damage to powdery mildew
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0051] Example 1 Allelic detection and multi-species identification of gourd head, tooth rough and white gourd
[0052] In view of the fact that the powdery mildew resistance genes Pm24 (dental grass) and Pm24b (white gourd) from Chinese wheat landraces have been mapped on the wheat 1DS chromosome, in order to clarify the powdery mildew resistance genes (named MlHLT) from the local variety Hulutou Whether it is located at the same gene locus as Pm24 and Pm24b, the hybrid combinations among these three landraces were prepared respectively, and the effect on Cucurbitum × Dentalia (4,517 F 2 single plant), gourd head × white gourd (4,318 F 2 single plant), tooth rough × gourd head (4,382F 2 single plant), Dentalia × white gourd (4,590 F 2 single plant) and white gourd×cucurbit head (4,692 F 2 single plant) of F 2 The artificial inoculation identification of the powdery mildew strain E09 at the seedling stage was carried out on a single plant, and it was found that all hybrid ...
Embodiment 2
[0054] The fine mapping of embodiment 2 powdery mildew resistance gene
[0055] Powdery mildew resistance identification of wheat landrace Hulutou from Shaanxi, China and wheat variety Shi 4185 from Hebei, China, DAB staining and trypan blue staining of wheat leaves 24 hours and 48 hours after inoculation, respectively, and The wheat leaves 7 days after inoculation were observed, and the results were as follows: figure 1 As shown, it was found that Hulutou had immune level resistance to BgtE09 prevalent in Beijing area, and Shi 4185 showed high sensitivity to BgtE09. Construct the second-generation hybrid population of Hulutou and Shi 4185, and its 310 F 2∶3 Families were identified for disease resistance, 71 F 2∶3 The family showed homozygous disease resistance, 150 F 2∶3 The pedigree showed resistance and sense separation, while F 2 Generation of 89 susceptible individual F 2∶3 All the families showed susceptibility, according to their F 2∶3 The resistance-sensitivity...
Embodiment 3
[0057] Confirmation of embodiment 3 powdery mildew resistance gene Pm24 candidate gene
[0058] There are 6 candidate genes in the physical interval described in Example 2 in the genome sequence of AL8 / 78 and Chinese spring, including 2 genes encoding receptor kinases (RLK1, RLK2), and 1 coding string A gene for cascade kinase (WTK), a gene encoding a CC-NBS-LRR domain protein (CNL), a gene encoding a hypothetical protein (HP) and a gene encoding a 50S ribosomal protein (RP) ( figure 2 shown in d and e). Analysis of the genome sequences of 6 candidate genes (RLK1, WTK, RLK2, CNL, HP and RP) in a disease-resistant wheat variety (Hulutou) and a susceptible wheat variety (Shi 4185) showed that only two genes, WTK and CNL Genome sequences of genes differed between the two wheat varieties ( figure 2 Shown in f and g), WTK and CNL were initially identified as candidate genes.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More