Kit for improving GPC3 detection sensitivity and preparation method thereof

A technology of detection sensitivity and kit, which is applied in the field of kits for improving GPC3 detection sensitivity and its preparation, can solve the problem of low sensitivity of kits, achieve the effect of expanding the detection linear range and improving detection sensitivity

Active Publication Date: 2020-12-01
WUHAN LIFE ORIGIN BIOTECH LTD
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the technical problem that the sensitivity of the existing GPC3 detection kit is not high, the invention provides a test kit for improving the detection sensitivity of GPC3, which has a higher detection sensitivity of GPC3 and a wider linear range

Method used

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  • Kit for improving GPC3 detection sensitivity and preparation method thereof
  • Kit for improving GPC3 detection sensitivity and preparation method thereof
  • Kit for improving GPC3 detection sensitivity and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 14

[0102] Example 14 and the R&D kit simultaneously detect samples with a concentration of 10-19860pg / mL. The sample concentration has been detected with a contrast reagent, and the concentration exceeding the detection range of the contrast reagent (36-4965pg / mL) is theoretically calculated based on the dilution ratio concentration.

[0103] Table 6 Example 14 and comparison reagent comparison result

[0104] Sample concentration pg / mL 0D value measured by R&D kit Embodiment 14 records photon value 0 0.115 3256 10 / 4256 20 0.113 7156 36 0.116 13156 72 0.149 26123 162 0.202 46411 360 0.288 119654 635 0.431 211563 1246 0.81 401236 2489 1.457 798456 4965 2.396 1612354 9930 3.265 3256471 19860 3.468 6598731 10 / 0 SNR / 1.31 36 / 0 SNR 1.01 4.04 4965 / 2489 SNR 1.64 2.02 19860 / 9930 SNR 1.06 2.03 (36-4965)r 0.9918 0.9999 (36-19860)r 0.8049 0.9999

[0105]...

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Abstract

The invention provides a kit for improving GPC3 detection sensitivity and a preparation method thereof, and belongs to the technical field of immunodetection; the kit comprises a reagent M and a reagent R; the reagent M is prepared by diluting a magnetic bead particle coupled mouse anti-human GPC3 monoclonal antibody, and the diameter of the magnetic bead particle is 0.8-3[mu]m; the diluent of themagnetic bead particle coupled mouse anti-human GPC3 monoclonal antibody comprises the following components: Procline 300, a buffer solution, a surfactant and protein; and the reagent R is prepared by diluting a rabbit anti-human GPC3 polyclonal antibody marked by alkaline phosphatase. The kit has the advantages of higher GPC3 detection sensitivity and wider linear range. The invention also provides a preparation method of the kit for improving the GPC3 detection sensitivity.

Description

technical field [0001] The invention belongs to the technical field of immune detection, and relates to a kit for improving the detection sensitivity of GPC3 and a preparation method thereof. Background technique [0002] Glypican 3 / Glypican-3 (GPC3) is a member of the Glypican family and belongs to the membrane heparan sulfate polysaccharide protein. In the embryonic stage of mammals, GPC3 plays an important regulatory role in the development of tissues and organs by affecting multiple molecular signaling pathways, and its loss of function will lead to excessive growth and deformation syndrome (SGBS). The abnormal expression of adult GPC3 is closely related to the occurrence and development of various tumors. GPC3 is up-regulated in hepatocellular carcinoma (HCC), which is related to the biological characteristics of HCC, and has a good application prospect in the early diagnosis, biological treatment and prognosis evaluation of liver cancer. [0003] GP03 has been confir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574G01N33/577G01N33/543G01N33/535G01N33/58
CPCG01N33/68G01N33/57438G01N33/57488G01N33/577G01N33/54326G01N33/54393G01N33/535G01N33/581G01N2333/705Y02A50/30
Inventor 李兰芝来祥兵舒芹赵愿安
Owner WUHAN LIFE ORIGIN BIOTECH LTD
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