Kit for improving GPC3 detection sensitivity and preparation method thereof
A technology of detection sensitivity and kit, which is applied in the field of kits for improving GPC3 detection sensitivity and its preparation, can solve the problem of low sensitivity of kits, achieve the effect of expanding the detection linear range and improving detection sensitivity
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Embodiment 14
[0102] Example 14 and the R&D kit simultaneously detect samples with a concentration of 10-19860pg / mL. The sample concentration has been detected with a contrast reagent, and the concentration exceeding the detection range of the contrast reagent (36-4965pg / mL) is theoretically calculated based on the dilution ratio concentration.
[0103] Table 6 Example 14 and comparison reagent comparison result
[0104] Sample concentration pg / mL 0D value measured by R&D kit Embodiment 14 records photon value 0 0.115 3256 10 / 4256 20 0.113 7156 36 0.116 13156 72 0.149 26123 162 0.202 46411 360 0.288 119654 635 0.431 211563 1246 0.81 401236 2489 1.457 798456 4965 2.396 1612354 9930 3.265 3256471 19860 3.468 6598731 10 / 0 SNR / 1.31 36 / 0 SNR 1.01 4.04 4965 / 2489 SNR 1.64 2.02 19860 / 9930 SNR 1.06 2.03 (36-4965)r 0.9918 0.9999 (36-19860)r 0.8049 0.9999
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