Unlock instant, AI-driven research and patent intelligence for your innovation.

Pesticide residue detection method

A pesticide residue detection and pesticide technology, applied in the detection field, can solve the problems of expensive instruments, long time-consuming, and high detection costs, and achieve the effects of reducing detection costs, fast and convenient processes, and low-cost detection.

Pending Publication Date: 2020-12-04
QINGHAI UNIVERSITY
View PDF9 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the existing method has the advantages of high sensitivity and low detection limit, it is difficult to meet the current situation of frequent food safety problems due to expensive instruments, long time consumption, and high detection cost.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for detecting pesticide residues, comprising the steps of:

[0025] S1. Construct a pesticide residue detection model based on the historical pesticide residue detection spectral data and the corresponding pesticide residue detection results;

[0026] S2. After the sample to be tested is ultrafinely pulverized, put it in an enzymatic hydrolysis tank, add neutral protease at a mass percentage of 2%, and cellulase at a mass percentage of 1%, enzymolyze at 30°C for 2 hours, and filter , to obtain enzymatic solution;

[0027] S3. Quickly freeze the obtained enzymatic solution and then thaw naturally to age and precipitate the starch in the extract; collect the supernatant, centrifuge the remaining part, collect the centrifuged liquid and merge it into the supernatant, inactivate the enzyme, and obtain the test solution ;

[0028] S4. Soak the imprinted hydrogel in the solution to be tested obtained in step S3, and after 0.1 MPa negative pressure treatment for 1 m...

Embodiment 2

[0032] A method for detecting pesticide residues, comprising the steps of:

[0033] S1. Construct a pesticide residue detection model based on the historical pesticide residue detection spectral data and the corresponding pesticide residue detection results;

[0034] S2. After the sample to be tested is ultrafinely pulverized, put it in an enzymatic hydrolysis tank, add neutral protease at a mass percentage of 3%, and cellulase at a mass percentage of 2%, enzymolyze at 50°C for 1 hour, and filter , to obtain enzymatic solution;

[0035] S3. Quickly freeze the obtained enzymatic solution and then thaw naturally to age and precipitate the starch in the extract; collect the supernatant, centrifuge the remaining part, collect the centrifuged liquid and merge it into the supernatant, inactivate the enzyme, and obtain the test solution ;

[0036] S4. Soak the imprinted hydrogel in the solution to be tested obtained in step S3, and after 0.3 MPa negative pressure treatment for 1 mi...

Embodiment 3

[0040] A method for detecting pesticide residues, comprising the steps of:

[0041] S1. Construct a pesticide residue detection model based on the historical pesticide residue detection spectral data and the corresponding pesticide residue detection results;

[0042] S2. After the sample to be tested is ultrafinely pulverized, put it in an enzymatic hydrolysis tank, add neutral protease at a mass percentage of 2.5%, add cellulase at a mass percentage of 1.5%, and enzymatically hydrolyze at 40°C for 1.5 hours. Filter to obtain the enzymatic solution;

[0043] S3. Quickly freeze the obtained enzymatic solution and then thaw naturally to age and precipitate the starch in the extract; collect the supernatant, centrifuge the remaining part, collect the centrifuged liquid and merge it into the supernatant, inactivate the enzyme, and obtain the test solution ;

[0044] S4. Soak the imprinted hydrogel in the solution to be tested obtained in step S3, and after 0.2 MPa negative press...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a pesticide residue detection method. The method comprises the following steps of constructing a pesticide residue detection model based on historical pesticide residue detection spectral data and corresponding pesticide residue detection results, carrying out superfine grinding on a sample to be detected, carrying out enzymolysis, filtering, quickly freezing the obtained enzymatic hydrolysate, naturally unfreezing, collecting the supernatant, centrifuging the remaining part, collecting the centrifugate, merging the centrifugate into a supernatant, and carrying out enzyme deactivation to obtain a solution to be detected, soaking the imprinted hydrogel in the obtained solution to be detected, carrying out negative pressure treatment for 1 min, instantly recovering tonormal pressure, taking out the imprinted hydrogel, and rinsing with deionized water to remove excess supernatant, and detecting the obtained imprinted hydrogel by using a portable Raman spectrometer, and inputting the obtained spectrum into the pesticide residue detection model to obtain a pesticide residue detection result of the to-be-detected sample. The pesticide residue condition in fruitsand vegetables can be rapidly detected with low cost.

Description

technical field [0001] The invention relates to a detection method, in particular to a pesticide residue detection method. Background technique [0002] Pesticides play a significant role in promoting the development of forestry and animal husbandry, but the environmental pollution and human health problems caused by the simultaneous use of pesticides are becoming more and more serious. The hazards of pesticide residues have also attracted more and more attention, and many countries have established pesticide residue limits in food. [0003] At present, the detection methods of pesticide residues in fruits and vegetables include: thin layer chromatography, gas chromatography, high performance liquid chromatography, supercritical fluid chromatography and so on. Although the existing methods have the advantages of high sensitivity and low detection limit, they are difficult to meet the current situation of frequent food safety problems due to expensive instruments, long time-...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/65G01N1/40
CPCG01N21/65G01N1/405
Inventor 李玮沈硕陈红雨
Owner QINGHAI UNIVERSITY