Device and method for preparing monodisperse calcium alginate microspheres and application

A calcium alginate, monodisperse technology, applied in the direction of microsphere preparation, gel preparation, microcapsule preparation, etc., can solve the problems of removal and cleaning, and achieve the effect of full reaction, convenient operation and simple structure

Active Publication Date: 2020-12-08
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method forms spherical particles, there are problems such as removing and washing the organic phase when collecting the gel microspheres.

Method used

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  • Device and method for preparing monodisperse calcium alginate microspheres and application
  • Device and method for preparing monodisperse calcium alginate microspheres and application
  • Device and method for preparing monodisperse calcium alginate microspheres and application

Examples

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Effect test

preparation example Construction

[0039] In some typical embodiments, the preparation method of the monodisperse calcium alginate microspheres comprises the following steps:

[0040] Step 1: Put the prepared outer water phase into the outer glass tube, when the outer water phase fluid flows out of the outer glass tube outlet, put the middle oil phase into the middle phase glass tube, and when the middle oil phase flows out of the middle phase glass tube outlet, Pass the inner water phase into the inner phase glass tube;

[0041] Step 2: Due to the effect of fluid shear force, the inner water phase forms a single-layer droplet covered by the intermediate oil phase at the outlet of the inner-phase glass tube, and the intermediate oil phase containing the single-layer droplet is in the middle-phase glass tube. A double-layer droplet covered by the outer water phase is formed at the outlet, and the double-layer droplet flows into the collected water phase configured in the collection petri dish along with the oute...

Embodiment 1

[0056] A device for preparing monodisperse calcium alginate microspheres, such as figure 1 As shown, it includes an inner phase glass tube 5, a middle phase glass tube 6 and an outer phase glass tube 7 arranged coaxially. The inner phase glass tube passes through the second dispensing needle and is inserted into the middle phase glass tube. The middle phase glass tube Insert the outer phase glass tube through the third dispensing needle, the outer phase glass tube extends into the collection culture dish, the inner phase glass tube communicates with the inner cavity of the first dispensing needle 2, and the middle phase glass tube communicates with the second point The inner space of the glue needle 3 communicates, the outer glass tube communicates with the inner space of the third glue dispensing needle 4, and the first glue dispensing needle, the second glue dispensing needle and the third glue dispensing needle are fixedly connected to the base plate.

Embodiment 2

[0058] A device for preparing monodisperse calcium alginate microspheres, the same as in Example 1, the difference is that the first dispensing needle, the second dispensing needle and the third dispensing needle are respectively connected to a syringe, and the syringe and the flow pump connected, use the flow pump to drive the respective syringes, so that the inner water phase, the middle oil phase and the outer water phase are respectively injected into the first dispensing needle, the second dispensing needle and the third dispensing needle.

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Abstract

The invention relates to a device and a method for preparing monodisperse calcium alginate microspheres and application. The device comprises an inner-phase glass tube, a middle-phase glass tube and an outer-phase glass tube which are coaxially arranged, the inner-phase glass tube penetrates through a second dispensing needle head to be inserted into the middle-phase glass tube, the middle-phase glass tube penetrates through a third dispensing needle head to be inserted into the outer-phase glass tube, the outer-phase glass tube extends into a collection culture dish, the inner-phase glass tube is communicated with an inner cavity of the first dispensing needle head, the middle-phase glass tube is communicated with an inner space of the second dispensing needle head, the outer-phase glasstube is communicated with an inner space of the third dispensing needle head, and the first dispensing needle head, the second dispensing needle head and the third dispensing needle head are fixedly connected with the bottom plate. The device and the method can be used for preparing the calcium alginate microspheres with better sphericity.

Description

technical field [0001] The invention relates to the technical field of preparation of calcium alginate microspheres, in particular to a device, method and application for preparing monodisperse calcium alginate microspheres. Background technique [0002] The statements herein merely provide background information related to the present invention and do not necessarily constitute prior art. [0003] Calcium alginate gel microspheres have good biocompatibility and biodegradability, and are widely used in encapsulating cells, drug carriers, etc., and their better spherical shape is crucial to their performance. Microfluidic technology can prepare microspheres with good monodispersity and sphericity. At present, the common method for preparing calcium alginate gel particles is to use oil-water two-phase to generate monodisperse sodium alginate droplets, and collect them in In the solution of the coupling agent, the inventors found that in this method, when the droplet crosses t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02B01J13/14
CPCB01J13/0052B01J13/02B01J13/14
Inventor 满佳夏荷李建勇张松李剑峰李东海
Owner SHANDONG UNIV
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