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Near-infrared fluorescent molecular probe for detecting aspartyl aminopeptidase, preparation method and use

A technology of aspartyl aminopeptidase and fluorescent molecular probe, which is applied in the fields of fluorescence/phosphorescence, chemical instruments and methods, and material analysis through optical means, and can solve the problems of inability to reflect enzyme activity and enzyme inactivation, etc. Achieve good cell imaging effect, low biological toxicity, and good response effect

Active Publication Date: 2022-04-12
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional method of detecting aspartyl aminopeptidase needs to extract and purify aspartyl aminopeptidase before testing, which will lead to the inactivation of the enzyme during the extraction process, so that the detection result can only reflect the amount of the extracted enzyme and not Does not reflect enzyme activity

Method used

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  • Near-infrared fluorescent molecular probe for detecting aspartyl aminopeptidase, preparation method and use
  • Near-infrared fluorescent molecular probe for detecting aspartyl aminopeptidase, preparation method and use
  • Near-infrared fluorescent molecular probe for detecting aspartyl aminopeptidase, preparation method and use

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Effect test

Embodiment 1

[0034] This embodiment detects the preparation of aspartyl aminopeptidase near-infrared fluorescent probe:

[0035] Add N-tert-butoxycarbonyl-D-aspartic acid 1-tert-butyl ester into the round bottom flask, slowly add dichloromethane under the condition of ice bath, and then stir; after 10 minutes in ice bath, keep the ice bath And add 2-(7-azabenzotriazole)-N,N,N',N'-tetramethyluronium hexafluorophosphate and N,N-diisopropylethylamine, stir for 30min and then add Compound 1. Then, keep stirring, remove the ice bath, and return to room temperature naturally; after a period of reaction at room temperature, the solution turns blue, and then filters with a sand core funnel to get the filtrate. Under ice-bath conditions, trifluoroacetic acid was slowly added to the filtrate, and stirred while adding. After adding trifluoroacetic acid, the ice bath was removed, and the room temperature was naturally restored. After a period of reaction, a large amount of insoluble matter appeared ...

Embodiment 2

[0041] This embodiment detects the preparation of aspartyl aminopeptidase near-infrared fluorescent probe:

[0042] Add N-tert-butoxycarbonyl-D-aspartic acid 1-tert-butyl ester into the round bottom flask, slowly add dichloromethane under the condition of ice bath, and then stir; after 10 minutes in ice bath, keep the ice bath And add 2-(7-azabenzotriazole)-N,N,N',N'-tetramethyluronium hexafluorophosphate and N,N-diisopropylethylamine, stir for 30min and then add Compound 1. Then, keep stirring, remove the ice bath, and return to room temperature naturally; after a period of reaction at room temperature, the solution turns blue, and then filters with a sand core funnel to get the filtrate. Under ice-bath conditions, trifluoroacetic acid was slowly added to the filtrate, and stirred while adding. After adding trifluoroacetic acid, the ice bath was removed, and the room temperature was naturally restored. After a period of reaction, a large amount of insoluble matter appeared ...

Embodiment 3

[0047] This embodiment detects the preparation of aspartyl aminopeptidase near-infrared fluorescent probe:

[0048] Add N-tert-butoxycarbonyl-D-aspartic acid 1-tert-butyl ester into the round bottom flask, slowly add dichloromethane under the condition of ice bath, and then stir; after 10 minutes in ice bath, keep the ice bath And add 2-(7-azabenzotriazole)-N,N,N',N'-tetramethyluronium hexafluorophosphate and N,N-diisopropylethylamine, stir for 30min and then add Compound 1. Then, keep stirring, remove the ice bath, and return to room temperature naturally; after a period of reaction at room temperature, the solution turns blue, and then filters with a sand core funnel to get the filtrate. Under ice-bath conditions, trifluoroacetic acid was slowly added to the filtrate, and stirred while adding. After adding trifluoroacetic acid, the ice bath was removed, and the room temperature was naturally restored. After a period of reaction, a large amount of insoluble matter appeared ...

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Abstract

The invention discloses a near-infrared fluorescent molecular probe for detecting aspartyl aminopeptidase. The molecular formula of the probe is C 35 h 36 N 3 o 4 + , the structural formula is as follows. The probe uses N-terminal aspartic acid as the response group to detect aspartyl aminopeptidase with excellent selectivity and good response effect, and its near-infrared absorption and fluorescence emission characteristics make it very good Applied to in vivo imaging, both absorption and emission have strong biological penetration in the near-infrared region, and can also reduce the interference of biological autofluorescence, and the detection is efficient.

Description

technical field [0001] The invention relates to a fluorescent molecular probe, a preparation method and an application thereof, in particular to a near-infrared fluorescent molecular probe for detecting aspartyl aminopeptidase. Background technique [0002] Aspartyl aminopeptidase belongs to the matrix metalloproteinase 18 family and is widely distributed in organisms. It regulates the activity of biologically active peptides by hydrolyzing N-terminal aspartic acid, thereby participating in various physiological or pathological processes, such as angiogenesis , hydrolytic balance, blood pressure, etc. Abnormal aspartyl aminopeptidase concentrations in organisms are often associated with some cancers, including brain, rectal and breast cancers. In order to clarify the role of aspartyl aminopeptidase in the occurrence and development of tumors, it is necessary to monitor its level in vivo in real time. The current detection methods for aspartyl aminopeptidase are mainly west...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D405/06C09K11/06G01N21/64
CPCC07D405/06C09K11/06G01N21/6428C09K2211/1029C09K2211/1088
Inventor 刘熠柳旺旺阿力亚·铁木尔
Owner CHINA PHARM UNIV