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A Paenibacillus polymyxa bfp-1 strain, biological preparations and applications

A polymyx spore-like, BFP-1 technology, applied in the directions of biocides, biochemical equipment and methods, chemicals for biological control, etc. Low requirements and low impact on the ecological environment

Active Publication Date: 2022-05-17
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defect of difficult prevention and control of orchid leaf spot disease, the present invention provides a Paenibacillus polymyxa BFP-1 strain, biological preparation and application

Method used

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  • A Paenibacillus polymyxa bfp-1 strain, biological preparations and applications
  • A Paenibacillus polymyxa bfp-1 strain, biological preparations and applications
  • A Paenibacillus polymyxa bfp-1 strain, biological preparations and applications

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Effect test

Embodiment 1

[0043] The antibacterial activity of embodiment 1 Paenibacillus polymyxa (Paenibacillus polymyxa) BFP-1 strain culture fluid to orchid leaf spot bacterium

[0044] 1. Experimental method

[0045] The activity of the strain culture solution (containing living bacteria and strain culture filtrate) was determined by plate confrontation method.

[0046] Bacterial strains were inoculated in LB culture medium, and cultured with shaking at 37°C and 180r / min for 48h. Use a hole puncher with an inner diameter of 6 mm to punch a hole in the center of the PDA plate, add 60 μL of bacterial solution into the hole, and inoculate the bacteria blocks of Phytophthora orchidum leaf spot on both sides of the hole 3 cm away.

[0047] 2. Experimental results

[0048] After determination, the plate confrontation test diagram of the inhibitory effect of the Paenibacillus polymyxa BFP-1 strain culture solution on the orchid leaf spot bacterium is as follows figure 2 As shown, it can be seen that th...

Embodiment 2

[0049] The antibacterial spectrum of embodiment 2 Paenibacillus polymyxa BFP-1 bacterial strain culture fluid

[0050] 1. Experimental method

[0051] The antibacterial spectrum of Paenibacillus polymyxa BFP-1 strain was determined by plate confrontation method with several flower fungal disease pathogens as objects.

[0052] Bacterial strains were inoculated in LB culture medium, and cultured with shaking at 37°C and 180r / min for 48h. Use a hole puncher with an inner diameter of 0.6 mm to punch a hole on the PDA medium, add 60 μL of bacterial solution into the hole, and inoculate Phytophthora sp., Fusarium oxysporum and Cylindrocladium spathiplylli. Taking the addition of LB culture medium as a control, each treatment was repeated 3 times, and cultured in a constant temperature incubator at 28 °C. When the diameter of the control colony reached about 5 cm, the width of the inhibition zone was measured.

[0053] 2. Experimental results

[0054] The plate confrontation test...

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Abstract

The invention discloses a Paenibacillus polymyxa BFP‑1 strain, a biological preparation and application. The present invention isolated and screened a Paenibacillus polymyxa BFP‑1 strain, which was deposited in the Guangdong Microbial Culture Collection Center on April 7, 2020, with the preservation number GDMCC No: 60996. The Paenibacillus polymyxa BFP‑1 strain provided by the present invention has significant inhibitory effect on orchid leaf spot fungus, requires low culture conditions, and has significant antibacterial effect on Phytophthora, Fusarium oxysporum or brown rot fungus, Moreover, the biological preparation obtained by culturing the bacterial strain in a liquid medium has a significant inhibitory effect on orchid leaf spot bacteria, and the source is environmentally friendly and non-toxic, has little impact on the ecological environment, and has a good application prospect.

Description

technical field [0001] The invention relates to the technical field of biopesticides, in particular to a Paenibacillus polymyxa BFP-1 strain, biological preparations and applications. Background technique [0002] Orchid is a high-grade potted flower, and its production occupies an important position in my country's flower industry. In recent years, with the gradual expansion of the planting scale of orchids in my country, the diseases have become increasingly serious. Among them, orchid leaf spot is a fungal disease caused by Fusarium sp., which has seriously affected the production of orchids. [0003] Orchid leaf spot can not only harm Chinese orchids, but also hybrid orchids. The disease can damage the young and growing leaves of orchids, forming small dark brown spots on the leaves, and causing the leaves to die in severe cases. Orchid leaf spot disease not only affects the growth of orchid plants, but also reduces the ornamental value of plants, which leads to the r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/12
CPCC12N1/20A01N63/22C12R2001/12C12N1/205
Inventor 张云霞麦章龙陈灿钿游春平向梅梅
Owner ZHONGKAI UNIV OF AGRI & ENG
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