Cell nucleus operation method based on cell nucleus position dynamic drifting modeling

Abstract

The invention relates to a cell nucleus operation method based on cell nucleus position dynamic drifting modeling. The cell nucleus operation method comprises the following steps: calibrating three-dimensional distribution of a cell nucleus relative to a polar body in an off-line manner; obtaining a dynamic drifting track of the cell nucleus relative to the position of a microneedle through three-dimensional finite element modeling; determining dominant influence factors for cell nucleus position dynamic drifting by using a factional factorial design method; and fitting the relationship between microneedle intracellular movement parameters and cell nucleus position dynamic drifting track parameters, determining the microneedle track required for approaching the cell nucleus, and controlling the microneedle to move in the cell along the determined track and approach the cell nucleus by the controller so as to complete the corresponding operation. By utilizing the cell nucleus operationmethod provided by the invention to perform the nucleus removal operation, the intracellular motion track and the cytoplasm removal quantity can be automatically controlled. Compared with a manual nucleus removal operation, the cell nucleus operation method disclosed by the invention has the advantages that the cytoplasm removal quantity is reduced by 60% under the same nucleus removal success rate, and the cleavage rate of cloned domestic pig embryos is nearly doubled.

Description

technical field [0001] The invention belongs to the field of cell-level micromanipulation, and in particular relates to a cell nucleus manipulation method based on dynamic drift modeling of cell nucleus position. Background technique [0002] Oocyte nuclear manipulation is widely used in operations such as animal cloning and nuclear transgene injection. The premise of operating the oocyte nucleus is: move the operating tool such as a microneedle to a position suitable for operating the nucleus near the nucleus, and then perform subsequent operations. However, this task is difficult to accomplish because the oocyte nucleus is generally invisible, and the movement of the microneedles in the cell will cause cell deformation and cause dynamic drift of the nucleus position. [0003] Although the cell nucleus can be manipulated by staining the cell nucleus, the cell nucleus positioning and the movement control of the microneedle will not only be damaged due to the photobleaching ...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem that it is difficult to control the microneedle to move to a suitable position near the nucleus to operate the nucleus in the existing cell nucleus operation method, the present invention proposes a cell nucleus operation method based on the dynamic drift modeling of the cell nucleus position. Determine the three-dimensional distribution range of the nucleus relative to the polar body to estimate the initial position range of the nucleus, obtain the dynamic drift trajectory of the nucleus position and its dominant influe

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