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A kind of biological sample analysis method of spiro compound

A technology for spiro compounds and biological samples, applied in the field of biomedicine, can solve the problems of complicated operation, single biological matrix material, long analysis period, etc., and achieve the effect of a simple analysis method

Active Publication Date: 2021-06-15
苏州旭辉检测有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The detection method of the concentration of spirocyclic compounds in biological samples of the present invention has the problems of complicated operation, long analysis period and single material from biological matrix, and a biological sample analysis method of spirocyclic compounds is provided

Method used

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  • A kind of biological sample analysis method of spiro compound
  • A kind of biological sample analysis method of spiro compound
  • A kind of biological sample analysis method of spiro compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 detects the spiro compound concentration in the biological sample

[0040] (1) Standard curve solution preparation

[0041] Weigh 10.52 mg of the reference substance of the spiro compound, place it in a 100 ml volumetric flask, add acetonitrile to dissolve and dilute to the mark, shake well and prepare a standard solution of 0.1052 mg / ml, add acetonitrile again to dilute, and prepare a concentration of 1- 500ng / mL standard curve solution;

[0042] (2) Quality control solution preparation

[0043] Weigh 10.78 mg of spiro compound reference substance, place it in a 100 ml volumetric flask, add acetonitrile to dissolve and dilute to the mark, shake well and prepare a 0.1078 mg / ml quality control solution, add acetonitrile again to dilute, and prepare the concentration to be 2 , 40, 400ng / mL quality control solution;

[0044] (3) Internal standard solution preparation

[0045] Weigh 11.43mg of terfenadine reference substance, place it in a 100ml volumetric ...

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Abstract

The invention provides a method for analyzing biological samples of spiro compounds, which is to analyze the spiro compounds through an efficient, fast and sensitive liquid phase-mass spectrometry system (LC-MS) method. This method adopts a simple organic solvent protein precipitation method to pre-treat biological samples. Based on the peak area ratio of the analyte and the internal standard, a standard curve is obtained by normalization, thereby quantitatively obtaining the concentration of spiro compounds in biological samples. This method provides a simple analytical method for the detection of spirocyclic compounds in the pharmacokinetics of spirocyclic compounds in animals.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for analyzing biological samples of spirocyclic compounds. Background technique [0002] Farnesoid X receptor (FXR) is a nuclear receptor and a sensor of bile acid, which can regulate the synthesis of bile acid and the flow of bile in the liver. Metabolic as well as inflammatory / immune responses play a role. Farnesoid X receptor (FXR) agonist has anti-cholestasis and anti-fibrosis effects. [0003] Recent studies have shown that spiro compounds can effectively treat diseases mediated by farnesoid X receptors. Compared with existing FXR agonists, they are more stable and have a longer half-life. It has potential application in the treatment of biliary cirrhosis, portal hypertension, nonalcoholic steatohepatitis, bile acid diarrhea, alcoholic hepatitis, primary sclerosing cholangitis or atherosclerosis. However, the current methods for detecting the concentration of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 李小川张枢石海兵李玉杰黄晓林
Owner 苏州旭辉检测有限公司