Graphene targeting drug carrier as well as preparation method and application thereof and transportation box

A graphene and transport box technology, applied in the field of nano-targeted drug carriers, can solve the problems of complex preparation process and poor targeting ability, and achieve the effects of simplifying the preparation process, improving the targeting ability and efficacy, and increasing the loading capacity

Pending Publication Date: 2020-12-18
NANJING MEDICAL UNIV
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Problems solved by technology

However, the existing nanomaterials used as targeting carriers still have dis

Method used

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  • Graphene targeting drug carrier as well as preparation method and application thereof and transportation box

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Embodiment 1

[0026] Example 1 Preparation of graphene-targeted drug carrier

[0027] 1.1 Preparation of biotinylated targeting substances

[0028] Dissolve and dilute the targeting recombinant anti-CD38 antibody with pH 8.0 0.1 mol / L carbonate buffer to obtain a dilution of targeting recombinant anti-CD38 antibody with a concentration of 1 mg / mL, and dissolve 1 mg of N-hydroxysuccinate in 1 mL of dimethyl sulfoxide Imide biotin to obtain N-hydroxysuccinimide biotin dilution, mix the targeted recombinant anti-CD38 antibody dilution and N-hydroxysuccinimide biotin dilution at a volume ratio of 1:0.2, and continue stirring for 3 hours , dialyzed with pH 8.0 0.1 mol / L phosphate buffer, and purified by molecular sieves to obtain biotinylated targeting recombinant anti-CD38 antibody;

[0029] 1.2 Preparation of magnetically aminated graphene oxide

[0030] Streptavidin and carboxy-modified nano-Fe 3 o 4 Shake and resuspend, add aminated graphene oxide at a volume ratio of 1:1.5, rotate and m...

Embodiment 2

[0035] Example 2 Preparation of graphene-targeted drug carrier

[0036] 2.1 Preparation of biotinylated targeting substances

[0037] Dissolve and dilute the targeting recombinant anti-CD38 antibody with pH 8.0 0.1 mol / L carbonate buffer to obtain a dilution of targeting recombinant anti-CD38 antibody with a concentration of 1 mg / mL, and dissolve 1 mg of N-hydroxysuccinate in 1 mL of dimethyl sulfoxide Imide biotin to obtain N-hydroxysuccinimide biotin dilution, mix the targeted recombinant anti-CD38 antibody dilution and N-hydroxysuccinimide biotin dilution at a volume ratio of 1:0.2, and continue stirring for 3 hours , dialyzed with pH 8.0 0.1 mol / L phosphate buffer, and purified by molecular sieves to obtain biotinylated targeting recombinant anti-CD38 antibody;

[0038] 2.2 Preparation of magnetically aminated graphene oxide

[0039] Streptavidin and carboxy-modified nano-Fe 3 o 4 Shake and resuspend, add aminated graphene oxide at a volume ratio of 1:1.5, rotate and m...

Embodiment 3

[0044] Example 3 Preparation of graphene-targeted drug carrier

[0045] 3.1 Preparation of biotinylated targeting substances

[0046] Dissolve and dilute the targeting recombinant anti-CD38 antibody with pH 8.0 0.1 mol / L carbonate buffer to obtain a dilution of targeting recombinant anti-CD38 antibody with a concentration of 1 mg / mL, and dissolve 1 mg of N-hydroxysuccinate in 1 mL of dimethyl sulfoxide Imide biotin to obtain N-hydroxysuccinimide biotin dilution, mix the targeted recombinant anti-CD38 antibody dilution and N-hydroxysuccinimide biotin dilution at a volume ratio of 1:0.2, and continue stirring for 3 hours , dialyzed with pH 8.0 0.1 mol / L phosphate buffer, and purified by molecular sieves to obtain biotinylated targeting recombinant anti-CD38 antibody;

[0047] 3.2 Preparation of magnetically aminated graphene oxide

[0048] Streptavidin and carboxy-modified nano-Fe 3 o 4 Shake and resuspend, add aminated graphene oxide at a volume ratio of 1:1.5, rotate and m...

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Abstract

The invention relates to the technical field of nano targeting drug carriers, in particular to a graphene targeting drug carrier as well as a preparation method and application thereof and a transportation box. The graphene targeting drug carrier comprises aminated graphene oxide, streptavidin and carboxyl modified nano Fe3O4, a biotinylation targeting substance and a load drug; the aminated graphene oxide is connected with the streptavidin and carboxyl modified nano Fe3O4 through covalent bonds; the load drug is connected with the aminated graphene oxide; and the biotinylation targeting substance is connected with the streptavidin and carboxyl modified nano Fe3O4. According to the graphene targeting drug carrier as well as the preparation method and application thereof and the transportation box, the streptavidin and carboxyl modified nano Fe3O4 is combined with the biotinylation targeting substance, so that the drug carrier not only has magnetic physical targeting capability, but also has active targeting capability of targeting substances such as a targeting antibody, an aptamer and the like, and the drug targeting capability is greatly improved. The aminated graphene oxide is used as a basic load unit, so that the load capacity is improved.

Description

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Claims

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Application Information

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Owner NANJING MEDICAL UNIV
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