Graphene targeting drug carrier as well as preparation method and application thereof and transportation box
A graphene and transport box technology, applied in the field of nano-targeted drug carriers, can solve the problems of complex preparation process and poor targeting ability, and achieve the effects of simplifying the preparation process, improving the targeting ability and efficacy, and increasing the loading capacity
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Embodiment 1
[0026] Example 1 Preparation of graphene-targeted drug carrier
[0027] 1.1 Preparation of biotinylated targeting substances
[0028] Dissolve and dilute the targeting recombinant anti-CD38 antibody with pH 8.0 0.1 mol / L carbonate buffer to obtain a dilution of targeting recombinant anti-CD38 antibody with a concentration of 1 mg / mL, and dissolve 1 mg of N-hydroxysuccinate in 1 mL of dimethyl sulfoxide Imide biotin to obtain N-hydroxysuccinimide biotin dilution, mix the targeted recombinant anti-CD38 antibody dilution and N-hydroxysuccinimide biotin dilution at a volume ratio of 1:0.2, and continue stirring for 3 hours , dialyzed with pH 8.0 0.1 mol / L phosphate buffer, and purified by molecular sieves to obtain biotinylated targeting recombinant anti-CD38 antibody;
[0029] 1.2 Preparation of magnetically aminated graphene oxide
[0030] Streptavidin and carboxy-modified nano-Fe 3 o 4 Shake and resuspend, add aminated graphene oxide at a volume ratio of 1:1.5, rotate and m...
Embodiment 2
[0035] Example 2 Preparation of graphene-targeted drug carrier
[0036] 2.1 Preparation of biotinylated targeting substances
[0037] Dissolve and dilute the targeting recombinant anti-CD38 antibody with pH 8.0 0.1 mol / L carbonate buffer to obtain a dilution of targeting recombinant anti-CD38 antibody with a concentration of 1 mg / mL, and dissolve 1 mg of N-hydroxysuccinate in 1 mL of dimethyl sulfoxide Imide biotin to obtain N-hydroxysuccinimide biotin dilution, mix the targeted recombinant anti-CD38 antibody dilution and N-hydroxysuccinimide biotin dilution at a volume ratio of 1:0.2, and continue stirring for 3 hours , dialyzed with pH 8.0 0.1 mol / L phosphate buffer, and purified by molecular sieves to obtain biotinylated targeting recombinant anti-CD38 antibody;
[0038] 2.2 Preparation of magnetically aminated graphene oxide
[0039] Streptavidin and carboxy-modified nano-Fe 3 o 4 Shake and resuspend, add aminated graphene oxide at a volume ratio of 1:1.5, rotate and m...
Embodiment 3
[0044] Example 3 Preparation of graphene-targeted drug carrier
[0045] 3.1 Preparation of biotinylated targeting substances
[0046] Dissolve and dilute the targeting recombinant anti-CD38 antibody with pH 8.0 0.1 mol / L carbonate buffer to obtain a dilution of targeting recombinant anti-CD38 antibody with a concentration of 1 mg / mL, and dissolve 1 mg of N-hydroxysuccinate in 1 mL of dimethyl sulfoxide Imide biotin to obtain N-hydroxysuccinimide biotin dilution, mix the targeted recombinant anti-CD38 antibody dilution and N-hydroxysuccinimide biotin dilution at a volume ratio of 1:0.2, and continue stirring for 3 hours , dialyzed with pH 8.0 0.1 mol / L phosphate buffer, and purified by molecular sieves to obtain biotinylated targeting recombinant anti-CD38 antibody;
[0047] 3.2 Preparation of magnetically aminated graphene oxide
[0048] Streptavidin and carboxy-modified nano-Fe 3 o 4 Shake and resuspend, add aminated graphene oxide at a volume ratio of 1:1.5, rotate and m...
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