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Simple method for quickly predicting whether water quality is biologically stable

A bio-stable and water-quality technology, applied in the preparation of test samples, material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of cumbersome measurement steps, and it takes a long time to determine the maximum growth value of bacteria, and achieves high promotion value. , the prediction method is simple and fast effect

Active Publication Date: 2020-12-18
EAST CHINA JIAOTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The MAP assay replaces the standard matrix carbon acetate in the traditional AOC bioassay with sodium dihydrogen phosphate, and replaces assimilable organic carbon with bioavailable phosphorus to evaluate the biological stability of water quality, which mainly reflects the impact of phosphorus sources on the biological stability of water quality. However, it still takes a long time to determine the maximum growth of bacteria, and the determination steps are cumbersome

Method used

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  • Simple method for quickly predicting whether water quality is biologically stable
  • Simple method for quickly predicting whether water quality is biologically stable
  • Simple method for quickly predicting whether water quality is biologically stable

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0048] Method Description

[0049] Measuring instruments, utensils and materials

[0050] Constant temperature incubator; sterile operating table; alcohol lamp; ATP tester; 40ml carbon-free sampling bottle;

[0051] Test bacteria

[0052] The bacterium used in the embodiment is P17 (Pseudomonas fluorescense) and NOX (Spirillum sp.)

[0053] test preparation

[0054] 1. Preparation of 1000╳standard carbon solution (the carbon concentration of acetic acid is 1000mg / L)

[0055] NaAc:5.67g / L

[0056] 2. Preparation of 1000╳buffer solution

[0057]

[0058] 3. Preparation of 2╳standard carbon solution (the carbon concentration of acetic acid is 2000μg / L)

[0059] 1000╳standard carbon solution: 2ml / L

[0060] 1000╳Buffer solution: 1ml / L

[0061] 4. Cultivation of inoculated bacterial mother solution in the test

[0062] Inoculate P17 and NOX colonies into 2╳ standard carbon solution for constant temperature cultivation at 30°C for about one month, and store them in cold ...

Embodiment 2

[0074] Refer to Example 1 for method description, measuring instruments, utensils and materials, etc.

[0075] This assay method specifically comprises the steps:

[0076] (1) Preparation of the test bacterial liquid: a specific bacterial culture liquid is used as the test bacterial liquid. Specific bacteria refer to bacteria that are in the maintenance growth stage after being cultured at room temperature for 1 month; the type of bacteria is NOX (Spirillum sp.).

[0077] (2) Preparation of water samples: use ultrapure water (JC) as a control water sample, and prepare pure water (CS) (pure water does not contain bacteria, so no conversion is required).

[0078] (3) Preparation of the inoculated water sample: add the same amount (50 μl of bacteria solution / ml of water sample) of the bacterial test solution to the water sample to be tested.

[0079] (4) ATP fluorescence detection: at room temperature, test inoculated pure water (CS) and inoculated ultrapure water (JC), record ...

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Abstract

A simple method for rapidly predicting whether water quality is biologically stable or not is characterized in that the method comprises the steps: equivalently and quantitatively adding specific bacteria into a to-be-detected water sample and pure water, carrying out ATP fluorescence detection, comparing detection results of the two: if a significant difference exists, predicting that water quality is biologically unstable, and if not, predicting that the water quality is stable. The prediction method is simple, convenient and fast, and the evaluation indexes are high in application and popularization value and practical.

Description

technical field [0001] The evaluation method of the invention can be applied to water supply treatment and water quality biological stability analysis of a drinking water pipe network, and belongs to the field of water supply and drainage monitoring of municipal engineering. Background technique [0002] The biostability of drinking water refers to the potential of biodegradable organic matter in drinking water to support the growth of heterotrophic bacteria, that is, when nutrients become the limiting factor for the growth of heterotrophic bacteria, the organic nutrients in water support the growth of bacteria with the greatest possibility, It can be expressed in terms of its equivalent concentration of nutrients that limit the growth of microorganisms. The harm caused by the bacterial regeneration problem in the drinking water distribution system mainly includes three aspects: the decrease of the water quality of the pipe network, the increase of pipe corrosion and the thr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/38
CPCG01N21/64G01N1/38
Inventor 王秋华张卫风许元龙王璐璐肖坤元高爱平龙肖霞
Owner EAST CHINA JIAOTONG UNIVERSITY