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Notopterygium root dampness-removing decoction fingerprint spectrum construction method and quality evaluation method

A technology of Qiang Huo Sheng wet soup and a construction method, which is applied in the field of construction of the fingerprint of Qiang Huo Sheng wet soup, can solve the problem of not being able to represent the overall quality of Qiang Huo Sheng wet soup, and achieves the goal of being beneficial to product quality control, avoiding single and one-sided, Precise and reproducible results

Active Publication Date: 2020-12-22
HARBIN ZHENBAO PHARMA +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The current quality control methods of Qianghuo Shengshi Decoction are mainly based on content determination, physical and chemical identification, TLC identification, etc., but the evaluation results of these methods cannot represent the overall quality of Qianghuo Shengshi Decoction

Method used

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  • Notopterygium root dampness-removing decoction fingerprint spectrum construction method and quality evaluation method
  • Notopterygium root dampness-removing decoction fingerprint spectrum construction method and quality evaluation method
  • Notopterygium root dampness-removing decoction fingerprint spectrum construction method and quality evaluation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] This embodiment provides a method for constructing the fingerprint of Notopterygium Shengshi Decoction, including:

[0086] The preparation of the reference solution: take isoimperatorin, dihydro-apebeinol angelate, 5-O-methyl visamidol glycoside, ammonium glycyrrhizinate, liquiritin, ferulic acid, osthole, An appropriate amount of reference substances such as Chuanxiong lactone I, etc., accurately weighed, dissolved in methanol and diluted to make each 1mL containing 4.0 μg of isoimperatorin, 3.5 μg of dihydro-epiperidinol angelate, 5-O-methylvesia A mixed solution of 3.8 μg mitanol glycoside, 3.7 μg ammonium glycyrrhizinate, 3.5 μg liquiritin, 4.0 μg ferulic acid, 4.0 μg osthole, and 0.03 μg ligustilide, shake well to obtain the product.

[0087] Preparation of the test solution: Take about 0.2 g of Qianghuo Shengshi Decoction granules (self-made), weigh it accurately, put it in a stoppered Erlenmeyer flask, accurately add 10 mL of methanol with a volume fraction of 6...

Embodiment 2

[0112] Adopt the method identical with embodiment 1 to prepare need testing solution, then use same chromatographic column, other test conditions are constant, only change flow rate and be 0.7mL / min, 0.9mL / min.

[0113] Such as Figure 10 Shown, the spectrogram of need testing solution in embodiment 1 and the spectrogram of embodiment 2 ( Figure 10 From top to bottom on the right side are the spectra corresponding to flow rates of 0.9mL / min, 0.8mL / min, and 0.7mL / min) for comparison, it can be seen that adjusting the flow rate up and down by 0.1 has no significant impact on the results. It shows that adjusting the flow rate has no obvious effect on the method.

Embodiment 3

[0115] Adopt the same method as in Example 1 to prepare the test solution, then use the same chromatographic column, and keep the other test conditions unchanged, only changing the column temperature to 35°C and 40°C.

[0116] Such as Figure 11 Shown, the spectrogram of need testing solution in embodiment 1 and the spectrogram of embodiment 3 ( Figure 11 From top to bottom on the right side are the spectra corresponding to the column temperature of 40°C, 30°C, and 35°C) for comparison, it can be seen that the adjustment of the column temperature has no obvious influence on the results. It shows that adjusting the column temperature has no obvious effect on the method.

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Abstract

The invention provides a notopterygium root dampness-removing decoction fingerprint spectrum construction method and a quality evaluation method. The notopterygium root dampness-removing decoction fingerprint spectrum construction method comprises the steps: respectively taking a test solution and a reference substance solution, carrying out high performance liquid chromatography determination toobtain a test spectrum and a reference substance spectrum, and processing the test spectrum by taking the reference substance spectrum as a reference to obtain a notopterygium root dampness-removing decoction fingerprint spectrum. The notopterygium root dampness-removing decoction quality evaluation method comprises the steps: obtaining the notopterygium root dampness-removing decoction fingerprint spectrum according to the construction method; according to chromatographic conditions used for constructing the notopterygium root dampness-removing decoction fingerprint spectrum, obtaining a to-be-detected sample spectrum by a high performance liquid chromatography; and carrying out similarity evaluation on the to-be-detected sample spectrum and the notopterygium root dampness-removing decoction fingerprint spectrum, and judging whether the to-be-detected sample is qualified or not according to the similarity. The notopterygium root dampness-removing decoction fingerprint spectrum obtained by the method can comprehensively and effectively evaluate the quality of a notopterygium root dampness-removing decoction.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicines, in particular to a method for constructing fingerprints of Notopterygium Shengshi Decoction and a quality evaluation method. Background technique [0002] Qianghuo Shengshi Decoction, the name of a traditional Chinese medicine prescription. It mainly includes notopterygium, solitary, ligusticum, fangfeng, licorice, vitex, chuanxiong and other medicinal materials. It is a damp-dispelling agent, which has the effects of expelling wind, eliminating dampness, and relieving pain; it is mainly used to treat rheumatism in the surface arthralgia syndrome. It is commonly used clinically to treat rheumatoid arthritis, rheumatoid arthritis, osteoporosis, ankylosing spondylitis and other rheumatism. [0003] The current quality control methods of Qianghuo Shengshi Decoction are mainly based on content determination, physical and chemical identification, and TLC identification, but the evaluatio...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/74G01N30/86
CPCG01N30/06G01N30/74G01N30/8686
Inventor 方同华闫久江陈玉梅董丽丽付英楠李晓波王博刘志东孙新茹赵涛赵薇夏迎迎
Owner HARBIN ZHENBAO PHARMA