Transgenic plants with increased yields

A gene and yield technology, applied in the field of transgenic plants with improved yield

Pending Publication Date: 2020-12-22
SEOUL NAT UNIV R&DB FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There has been some success in increasing seed yield by manipulating the DWF4 gene pathway or the BRI1 gene pathway, but more robust strategies are still needed

Method used

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  • Transgenic plants with increased yields
  • Transgenic plants with increased yields
  • Transgenic plants with increased yields

Examples

Experimental program
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Effect test

Embodiment 1

[0187] Generation and screening of high-yielding plant lines

[0188] This example describes methods for generating mutant plant lines and screening for high-yielding plants. Mutant plants produced by the methods of this example may exhibit increased growth characteristics, such as increased plant height, plant weight, number of tillers, number of spikes, number of seeds, weight of seeds, size of seeds, leaf angle, number of primary branches , number of secondary branches and / or growth rate. As disclosed herein, modulation of genes associated with DP-E2F signaling (eg, overexpression of the PZR1 gene in rice) using the methods of this example can result in high-yielding plants.

[0189] Cloning and Plant Transformation Vectors used to generate Oryza sativa and Arabidopsis plants overexpressing rice OsDPB / PZR1 under the control of the CaMV 35S promoter were constructed as follows: RNA was extracted and cDNA was synthesized from 7-day-old rice seedlings with specific primers (T...

Embodiment 2

[0197] Method for conferring resistance to plant lines to the BR biosynthesis inhibitor propiconazole

[0198] This example describes methods for generating plants or plant lines resistant to herbicides that affect brassinosteroid signaling. The generation of plants that are resistant to herbicides can be used in methods for producing high yielding crop fields. For example, a crop field containing herbicide-resistant crop plants can be treated with herbicides to effectively remove a weed or weeds from the crop field, thereby allowing the crop plants to thrive and increasing the overall Yield.

[0199] The response of rice to different concentrations of the inhibitor Pcz was studied to determine the optimal conditions for isolating Pcz-resistant mutants. Pcz affects rice plant growth and induces dwarfing in a dose-dependent manner ( Figure 8A with Figure 8B ). Treatment with 30 μM Pcz reduced the total size of wild-type seedlings by up to 47%, and the root response was eve...

Embodiment 3

[0203] Evaluation of Plant Architecture and Yield of Rice Plants

[0204] This example describes methods for evaluating plant architecture and yield in plants produced by genetic modification methods disclosed herein, such as those described in Example 1.

[0205] Examining the phenotypes of adult plants under field and greenhouse conditions, pzr1-D plants were found to have higher yields than wild-type plants ( Figure 2A to Figure 2I ). Total plant weight was 33% higher in the mutant than in the wild type, although their total height was not significantly different. The increase in weight could be explained by an increase in the number of tillers in the mutant, as well as an increase in the number of spikes per plant ( Figure 2A to Figure 2F ). Seed weight per plant increased from 30 g in the wild type to 50 g in the mutant, indicating an increase in yield of approximately 160%. The morphology of spikes was detected, and it was found that not only the number of spikes i...

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Abstract

Compositions and methods for increasing plant yield, either directly or through selective inhibition of competing weeds, are disclosed herein. Particular focus is paid to cell cycle signaling permutations such as mutations in the DP-E2F-Rb cell cycle regulatory pathway, and to permutations in brassinolide pathways as transgenic or small-molecule targets for increasing plant yield.

Description

[0001] cross reference [0002] This application claims the benefit of U.S. Provisional Patent Application No. 62 / 710,603, filed February 16, 2018, which is hereby incorporated by reference in its entirety for all purposes. Background technique [0003] Plant yield can be improved by manipulating the genetic background of the plant. For example, manipulation of a plant's genetic makeup can affect seed yield, plant height, leaf erection, number of tillers and ears per plant, and can affect seed size through manipulation of plant DNA. In the case of crop plants, increases in plant yield obtained through genetic modification can provide commercial and social advantages. Some success has been achieved in increasing seed yield by manipulating the DWF4 gene pathway or the BRI1 gene pathway, but more robust strategies are still needed. Contents of the invention [0004] This article describes methods for engineering traits of interest in plant cells. Some of the methods disclose...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82
CPCC07K14/415C12N15/8298C12N15/8274C12N15/8261Y02A40/146
Inventor 克劳迪娅·科瓦兰崔圣和
Owner SEOUL NAT UNIV R&DB FOUND
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