Limbal stem cell culture medium and culture method

Abstract

The invention provides a limbal stem cell culture medium and culture method. The culture medium comprises the following components: 8-10% of fetal bovine serum, 10-15 [mu]g / ml of madecassoside, 0.3-0.8 [mu]g / ml of follistatin related protein, 10-20 mg / ml of transferrin, 0.3-0.5 mg / ml of glutamic acid amine, 2-3 ml of 100 x penicillin-streptomycin mixed solution, 5-8 [mu]g / ml of insulin, 0.2-0.5 [mu]g / ml of hydrocortisone, 10-15 ng / ml of cholera toxin, 3-5 ng / ml of platelet-derived growth factor, and the balance DMEM / F12 (1:1). The limbal stem cell basal culture medium can better promote the growth of LSCs, up-regulates cell viability, improves cell proliferation activity, is good in stability, fully delays amplification ability exhaustion and inhibits differentiation of LSCs, and maintainsthe characteristics of LSCs.

Description

technical field [0001] The invention relates to the technical field of stem cell culture, in particular to a corneal limbal stem cell culture medium and a culture method. Background technique [0002] Limbal stem cells (LSCs) are the junction of the cornea, conjunctiva and sclera, and the healing of corneal epithelium is accomplished through the migration and proliferation of limbal stem cells. Corneal limbal stem cells play an important role in the reconstruction of the ocular surface. At present, limbal stem cells are isolated and cultured in vitro to obtain limbal stem cells for corneal transplantation to treat ocular surface diseases. This method overcomes and avoids some patients' own limbal stem cells. Limited access and some potential immune rejection. [0003] At present, during the in vitro culture of limbal stem cells, due to the limitations of the microenvironment created by different media for limbal stem cells, the existing limbal stem cells have slow prolifera...

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Problems solved by technology

[0003] At present, in the process of in vitro culture of limbal stem cells, the existing limbal stem cells have slow proliferation, low proliferation activity, high requirements for culture conditions, and poor stability due to the limitations of the microenvironment created by different culture media for limbal stem cells. Therefore, the preparation of a limbal stem cell culture medium that can better maintain the characteristics of limbal stem cells, delay the exhaustion of expansion ability and inhibit the differentiation process in the in vitro culture process is very important for achieving high-efficiency expansion of the number of limbal stem cells. It is of great significance to provide a rapid and stable cell source for the research on the specific mechanism of limbal stem cells and transplantation therapy

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