Dual-fluorescence PCR primers, probes, method and kit for identifying capripoxvirus and lumpy skin disease virus
A goat pox virus, sheep pox virus technology, applied in the field of probes, double fluorescent PCR primers, methods and kits, can solve the problem of inability to distinguish sheep pox virus and bovine nodular skin disease virus, unable to diagnose in time, and ineffective. Distinguish and other problems to achieve the effect of improving detection accuracy and precision, high accuracy and strong specificity
Active Publication Date: 2020-12-25
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI +1
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Abstract
The invention discloses dual-fluorescence PCR primers, probes, method and kit for identifying capripoxvirus and lumpy skin disease virus. The kit comprises two pairs of specific primers CaPV-130-F2 and CaPV-130-R2, and LSD-132-F5 and LSD-132-R6, and TaqMan-MGB probes, wherein 5'-end labeled report fluorescent dyes of CaPV-130-P2 and LSD-132-LP5 probes are FAM and VIC respectively, and 3'-end labeled fluorescence quenching groups are MGB. The kit provided by the invention can be used for detecting goat pox virus, sheep pox virus and lumpy skin disease virus in scabs, papules, vesicles, blood and other samples of sheep, goats and cattle, and can provide certain technical support and help for the diagnosis, epidemiological investigation, prevention and control and purification of capripoxvirus.
Application Domain
Microbiological testing/measurementMicroorganism based processes +1
Technology Topic
Pox virusEpidemiologic survey +9
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