Method for extracting shellfish DNA from shellfish blood
A shellfish and blood technology, which is applied in the field of DNA extraction, can solve the problems of shellfish mechanical damage, lethality, unfavorable shellfish species conservation, genetic breeding shellfish resource protection, etc., and achieve the effect of improving efficiency, obtaining more and being easy to obtain
Pending Publication Date: 2021-01-05
JIANGSU OCEAN UNIV
View PDF5 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
However, traditional sampling methods mostly focus on harmful sampling or non-destructive sampling, such as direct dissection, shell opening with a flare, etc., which cause strong mechanical damage or even death to the shellfish, which is not conducive to the later conservation of shellfish, Genetic breeding and protection of shellfish resources, especially for endangered and rare shellfish
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment
[0020] Example: see figure 1 , the present invention provides a technical solution: a method for extracting shellfish DNA from shellfish blood, the specific steps are as follows:
[0021] Step 1: Collect shellfish blood and pre-treatment; open a small hole of 0.3-0.4mm in the thick adductor muscle of shellfish, use a disposable sterile syringe to extract shellfish blood; transfer the collected blood to a 1.5mL centrifuge tube, After mixing, centrifuge at 800r / min for 3min, and take the supernatant;
[0022] Step 2: DNA extraction;
[0023] S21: Put the blood extracted in step 1 into a 1.5mL centrifuge tube, add 600μL lysate and 10μL proteinase K, take two 15mL centrifuge tubes, add 10mL lysis solution and 10μL proteinase K to each, and mix well with a pipette , add 610 mixed solution to each sample, and digest at 55°C overnight;
[0024] S22: Add chloroform and phenol into two 10mL large centrifuge tubes respectively, and add 300 μL of phenol and chloroform to each sample; ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a method for extracting shellfish DNA from shellfish blood. The method comprises the following steps of 1, collecting shellfish blood and carrying out pretreatment; and 2, extracting the DNA. The method mainly solves the problem that in the shellfish breeding process, shellfish parents die due to breeding conditions or other problems, and therefore samples are lost. A traditional method for extracting shellfish DNA needs destructive sampling, so that the same shellfish can only be sampled in one growth stage, blood samples of the shellfish can be extracted in differentgrowth stages through the method, DNA can be extracted in different stages, and the method has important research significance and practical value in the aspects of shellfish whole-genome selective breeding and the like. Experimental materials are easier to obtain, and blood can be repeatedly taken for multiple times; and compared with a traditional animal tissue sampling, the method has the advantages that a sample grinding process is reduced, so that the DNA extraction efficiency is greatly improved.
Description
technical field [0001] The invention relates to the field of DNA extraction, in particular to a method for extracting shellfish DNA from shellfish blood. Background technique [0002] Shellfish farming plays an important role in supplying high-quality protein, increasing fisherman's income, and improving the offshore environment. At the same time, shellfish farming is also facing the problem of resource depletion. More and more scholars use molecular biology techniques to obtain shellfish DNA and study its genetic information, and use molecular assisted breeding to improve shellfish quality and breed shellfish. The purpose of this kind of anti-stress new product is to solve the problem of depletion of shellfish resources. However, traditional sampling methods mostly focus on harmful sampling or non-destructive sampling, such as direct dissection, shell opening with a flare, etc., which cause strong mechanical damage or even death to the shellfish, which is not conducive to ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
CPCC12N15/1003
Inventor 董志国任国梁李德盛柳佳玲梁夏菲夏青崔振权
Owner JIANGSU OCEAN UNIV