Application of Vibrio alcohol compounds in the preparation of marine fouling biological control agents
A technology of biological control agent and vibrio alcohol, which is applied in biocides, biocide-containing paints, plant growth regulators, etc., to achieve good social benefits, inhibit marine organisms from adhering, and have great potential for popularization and application
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Embodiment 1
[0026] Experimental group: Dissolve quantitative Vibrio alcohol compounds in methanol with a solution concentration of 282.6 μg / mL, add 1 mL of the solution to a petri dish with a diameter of 6 cm, and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the content of the vibrio alcohol compound coated on the bottom of the petri dish is 10 μg / cm 2 . Add 13 mL of filter-sterilized seawater.
[0027] Control group: Add 1 mL of solvent methanol to make it evenly distributed on the bottom of the culture dish. After the solvent is completely evaporated, add 13 mL of filtered and sterilized seawater.
[0028] Blank group: Add 13 mL of filtered and sterilized seawater.
[0029] Four parallel samples were set up in each of the experimental group, the blank group and the control group. During the attachment test, the reticulated barnacle Venus larvae were randomly assigned to the blank group, the control group, and the experimental group. A...
Embodiment 2
[0034] Experimental group: the Vibrio alcohol compound was dissolved in methanol to prepare a solution with a concentration of 28.26 μg / mL. Add 1 mL of this solution to a petri dish with a diameter of 6 cm and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the content of the vibrio alcohol compound coated on the bottom of the petri dish is 1.0 μg / cm 2 . Add 13 mL of filter-sterilized seawater.
[0035] Control group: Add 1 mL of solvent methanol to make the solution evenly distributed on the bottom of the petri dish. After the solvent is completely evaporated, add 13 mL of filtered and sterilized seawater.
[0036] Blank group: Add 13 mL of filtered and sterilized seawater.
[0037] Determination of the number of larvae: Take the water body containing the larvae of the emerald mussel vectoli from the nursery pond and concentrate it with sieve silk. Take 50 mL each for three times, add 1-2 drops of formalin solution to kill th...
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