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Cell screening chip, cell screening system and method thereof

A cell and chip technology, applied in the field of cell screening chips, can solve the problems of easy rupture, low capture rate of target cells, lack of target cells, etc., and achieve the effect of high capture rate

Pending Publication Date: 2021-01-12
SHANGHAI FLUOTEK MEDICAL DEVICE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, due to the heterogeneity of specific antigens in different types of target cells in the former method, and even the absence of this specific antigen in some target cells, the capture rate of target cells is low; in the latter method, target cells are randomly Easily ruptures when buffer flows through microfilters, resulting in low capture of target cells

Method used

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  • Cell screening chip, cell screening system and method thereof
  • Cell screening chip, cell screening system and method thereof
  • Cell screening chip, cell screening system and method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0063] In order to separate the target cells in the sample solution for subsequent observation or inspection, an embodiment of the present disclosure provides a cell screening chip. The material of the cell screening chip can be a transparent material for subsequent identification of the target cells. refer to figure 1 , The cell screening chip in the embodiment of the present disclosure includes a chip body 100 and a cover 180 , the cover 180 is covered on the chip body for sealing the chip body 100 and preventing the sample solution from overflowing the cell screening chip.

[0064] The chip body 100 is provided with a sample inlet 110, a liquid inlet groove 120, a screening array 130 (such as figure 2 shown by the dotted line), the liquid outlet groove 140 and the sample outlet 150. refer to figure 2 , one end of the liquid inlet groove 120 communicates with the sample inlet 110 , and the extending direction of the liquid inlet groove 120 and the liquid outlet groove 14...

Embodiment 2

[0125] refer to Figure 21 , the embodiment of the present disclosure provides a cell screening system for isolating and identifying target cells. The cell screening system includes the above-mentioned cell screening chip 10 , a sampling pump 20 and a waste liquid collection device 70 . The sample inlet of the cell screening chip 10 is connected to the sample pump 20 , and the sample outlet is connected to the waste liquid collection device 70 . The sampling pump 20 is used to pump the sample solution, the cell screening chip 10 is used to capture the target cells, thereby separating the target cells from the sample solution, and the waste liquid collection device 70 is used to collect the waste that flows out from the cell screening chip 10. liquid.

[0126] The sample injection pump 20 includes a sample solution pump 22 , and the sample solution pump 22 pumps the sample solution into the cell screening chip 10 . In a possible example, the sample solution pump 22 includes ...

Embodiment 3

[0135] refer to Figure 22 , the embodiment of the present disclosure provides a cell screening method, which is applicable to the above cell screening system for isolating and identifying target cells, and the cell screening method includes:

[0136] S101 , sequentially injecting the surface treatment solution and the diluent into the cell screening chip 10 for pretreatment.

[0137] The surface treatment solution is pumped into the cell screening chip 10 through the injection pump 20, and flows out from the waste liquid collection device 70 to perform surface treatment on the functional area of ​​the cell screening chip 10 for capturing target cells. The surface treatment solution can be PVP.

[0138] Then pump the diluent into the cell screening chip 10 through the sample pump 20, rinse the surface treatment solution, fill the cell screening chip 10, and discharge air bubbles. The diluent can be PBS.

[0139] S102. Inject the sample solution containing the target cells int...

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Abstract

The invention provides a cell screening chip, a cell screening system and a method thereof, relates to the technical field of biological detection, and is used for solving the technical problem of lowcapture rate of target cells. A chip body of the cell screening chip is provided with a liquid inlet groove and a liquid outlet groove, the tail end of the liquid inlet groove is closed along the flow guide direction of the liquid inlet groove, and a screening array is formed between the side walls, close to each other, of the liquid inlet groove and the liquid outlet groove; each screening unitof the screening array comprises an accommodating cavity and a screening channel communicating with the accommodating cavity, the accommodating cavity communicates with the liquid inlet groove, and the screening channel communicates with the liquid outlet groove; the diameter of the target cells is larger than the width of the screening channel and smaller than the width of the accommodating cavity; and the included angle between the flow guide direction of the accommodating cavity and the flow guide direction of the liquid inlet groove is an acute angle, a sample solution in the liquid inletgroove stably flows into the accommodating cavity, the vortex and backflow of the sample solution are reduced, the probability that the target cells are deformed and extruded out of the screening channel due to impact is reduced, and the capture rate of the target cells is improved.

Description

technical field [0001] The disclosure relates to the technical field of biological detection, in particular to a cell screening chip, a cell screening system and a method thereof. Background technique [0002] In the field of biological detection, it is often necessary to separate target cells for further observation or inspection of target cells, for example, the separation and detection of circulating tumor cells. Circulating Tumor Cell (CTC) is a kind of tumor cell that comes from the primary tumor and enters the blood circulation system. These cells can develop into metastases through the blood circulation system, and then multiply to form secondary tumors. Therefore, Timely isolation and detection of such cells is of great significance for monitoring the treatment and recurrence of tumors. [0003] There are two main methods for the screening of target cells, one is the immunoaffinity capture method, which uses the affinity between the specific antigen of the target ce...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12N5/00
CPCC12M23/02C12M25/06C12N5/00
Inventor 杨旸庄紫云康磊
Owner SHANGHAI FLUOTEK MEDICAL DEVICE CO LTD