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Primers of InDel molecular marker related to rice grain length trait and application of primer

A rice grain and molecular marker technology, which is applied in the field of molecular genetics and breeding of crops to achieve the effects of good stability, saving production costs and controlling the size of breeding populations

Active Publication Date: 2021-01-12
NANJING AGRICULTURAL UNIVERSITY
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

Traditional conventional cross-breeding relies on manual visual phenotypic selection, which not only requires researchers to have rich experience, but also breeds for several years or even ten years

Method used

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  • Primers of InDel molecular marker related to rice grain length trait and application of primer
  • Primers of InDel molecular marker related to rice grain length trait and application of primer
  • Primers of InDel molecular marker related to rice grain length trait and application of primer

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Experimental program
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Effect test

Embodiment 1

[0035] Example 1 Application of InDel Molecular Marker TF01 in Identification of Rice Grain Length

[0036] (1) Preparation of primer TF01

[0037] The primer sequences shown in Table 1 below were artificially synthesized.

[0038] Table 1

[0039]

[0040] (2) Application of InDel molecular marker TF01 in identifying rice grain length

[0041] After sampling the single plant of the test plant at the seedling stage, its genome was extracted and used as a template to amplify TF01 with primers, and after the product was obtained, it was tested on a non-denaturing polyacrylamide gel with a concentration of 8%. According to the test results, according to The following method is used to determine the genotype of a single plant to be tested: if the amplified product of the rice to be tested has a band with a size of 131 bp, the rice to be tested is a candidate long-grained rice line.

[0042] The amplification process is as follows:

[0043] A total of 10 μl of amplification re...

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Abstract

The invention belongs to the field of crop molecular genetics and breeding, and provides primers of an InDel marker related to a rice grain length trait and application of the primers. The sequence ofa forward primer and the sequence of a reverse primer of the marker are 5'-CGGAGGTGATGGCAGGAG-3'and 5'-GTACTGCGTGTTGGAGCACC-3' respectively; polymerase chain reaction (PCR) amplification is conductedon genomic DNA of rice samples by utilizing the primers, and electrophoresis detection is conducted on amplified products, thus discovering that the grain length of a rice variety with the amplifiedband size of 131bp is obviously greater than that of a rice variety with the band size of 125bp. The primers have important application value for screening and breeding high-yield new rice varieties.

Description

technical field [0001] The invention belongs to the field of crop molecular genetics and breeding, and specifically provides molecular marker primers related to rice grain length and applications thereof. Background technique [0002] Rice (Oryza sativa L.) is the first food crop in China, and its stable yield is one of the important guarantees for national food security. With the impact of urbanization, water resource constraints, and planting structure adjustments, the actual planting area of ​​rice in my country has declined. Therefore, in order to meet the growing population's demand for food, it is of great significance to increase the unit yield of rice. [0003] The yield per plant of rice is determined by factors such as the number of panicles per plant, the number of grains per panicle and grain weight, and the grain weight of rice is determined by its grain size and grain filling degree. When the degree of grain filling is ideal, the grain weight of rice can be i...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 黄骥汤正宾高秀莹鲍永美张佳琪张红生
Owner NANJING AGRICULTURAL UNIVERSITY
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