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Preparation process of agricultural peptidoglycan derived from waste thalli

A preparation process, peptidoglycan technology, applied in the direction of nitrogen fertilizer, etc., can solve the problems of cumbersome methods and low utilization rate

Pending Publication Date: 2021-01-15
JINAN HANGCHEN BIOTECHNOLOGY CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a superfine pulverizer for rapid wall breaking, simple operation, high efficiency and easy industrial production in view of the cumbersome methods for preparing peptidoglycan and the low utilization rate of Staphylococcus nepalis bacteria. Method for agricultural use of powdered peptidoglycan

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The technological process of this embodiment is:

[0021] 1) Select 5 parts of sodium hydroxide, superfinely pulverize for 5 minutes to remove teichoic acid;

[0022] 2) Select 10 parts of sodium lauryl sulfate, ultrafinely pulverize for 30min to remove protein;

[0023] 3) The centrifugation speed of solid-liquid separation of aqueous solution is 8000rpm / min, and the centrifugation time is 10min;

[0024] 4) The yield of crude peptidoglycan was 56.23%

[0025] 5) Its protein content is 13.2%, and its neutral sugar content is 36.77%;

Embodiment 2

[0027] The technological process of this embodiment is:

[0028] 1) Select 5 parts of sodium hydroxide, ultrafinely pulverize for 5min to remove teichoic acid;

[0029] 2) Select 13 parts of sodium lauryl sulfate, ultrafinely pulverize for 30min to remove protein;

[0030] 3) The centrifugal speed of the solid-liquid separation of the aqueous solution is 8000rpm / min, and the centrifugal time is 20min;

[0031] 4) The yield of crude peptidoglycan was 53.34%

[0032] 5) Its protein content is 10.28%, and its neutral sugar content is 39.87%;

Embodiment 3

[0034] The technological process of this embodiment is:

[0035] 1) Select 6 parts of sodium hydroxide, ultrafinely pulverize for 5min to remove teichoic acid;

[0036] 2) Select 15 parts of sodium lauryl sulfate, ultrafinely pulverize for 30min to remove protein;

[0037] 3) The centrifugal speed of solid-liquid separation of aqueous solution is 8000rpm / min, and the centrifugation time is 20min;

[0038] 4) The yield of crude peptidoglycan was 52.65%

[0039] 5) Its protein content is 8.98%, and its neutral sugar content is 43.68%;

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Abstract

The invention discloses a preparation process of peptidoglycan derived from waste thalli dregs. The preparation process comprises the following steps: culturing seeds by virtue of Nipoisson bacteria,inoculating the seeds into glutamic acid waste liquid with relatively high salt content, obtaining thalli after fermentation is finished, and carrying out superfine grinding on the thalli to remove teichoic acid and protein. The production process does not need special equipment, is low in energy consumption and simple in process flow, and is an agricultural peptidoglycan preparation process whichis easy to industrialize and has good economic benefits.

Description

technical field [0001] The invention belongs to the field of agricultural fertilizers, and relates to a process for preparing agricultural peptidoglycan from discarded bacterial cells. Background technique [0002] Peptidoglycan (PG) is a component of bacterial cell wall, its basic structure is a heteropolysaccharide alternately linked by N-acetylglucosamine (N-Acetylglucosamine) and N-acetylmuramic acid (N-Acetylmuramic Acid) Macromolecules formed by cross-linking with peptides of different compositions. Staphylococcus naiporus is often used to ferment and prepare monosodium glutamate waste liquid to reduce sugar, and a large amount of solid bacterial residue will be produced after production. However, these bacterial residues contain more active components, among which peptidoglycan is the main component of the cell wall. Studies have shown that peptidoglycan has a variety of biological activities, such as anti-immune activity, growth promotion of vegetables and other ac...

Claims

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Application Information

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IPC IPC(8): C08B37/00C05C11/00
CPCC05C11/00C08B37/0003
Inventor 李昆仑赵林张峰李宝君赵晨苏乐岳秋林张娟
Owner JINAN HANGCHEN BIOTECHNOLOGY CO LTD
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