A Drosophila Eclosion Monitoring System

Abstract

The invention discloses a fruit fly eclosion monitoring system, which belongs to the technical field of life sciences and comprises a mechanical transmission device and a fruit fly eclosion monitoring device; The fruit fly eclosion monitoring device is sent to the culture bottle of fruit flies, and the fruit fly eclosion monitoring device is used to realize the real-time monitoring of the fruit fly's eclosion process; the fruit fly eclosion monitoring device is composed of a video sensor, a light source box and a monitoring device The video sensor is arranged on the light source box to collect the real-time eclosion state information of fruit flies, and transmit the state information to the monitoring device. The invention realizes the monitoring of the eclosion state of fruit flies through a video sensor, and sends a video of the state of fruit flies in the fruit fly culture bottle to the experimenter through the server and backs it up, so as to avoid missing the eclosion time of the fruit fly due to the negligence of the experimenter, thereby failing to timely Obtain sufficient effective experimental samples, and then affect the process and results of the experiment.

Description

technical field [0001] The invention belongs to the technical field of life sciences, and in particular relates to a fruit fly emergence monitoring system. Background technique [0002] Drosophila is an important model organism in the field of life sciences. Compared with other experimental animals, Drosophila has many advantages such as short generations, many offspring, clear genetic background, and mature techniques for genetic manipulation of individuals. Body resources are abundant. What is particularly striking is that the genes of Drosophila and mammals are highly conserved. Three-quarters of the human disease-related genes can find homologous genes in Drosophila, while 87 of the 282 human mental retardation genes % have homologous genes in Drosophila. This evolutionary conservation makes Drosophila an ideal model organism, and the results of its research may have certain reference significance for the research of mammals and even humans. Since Morgan began to use ...

AI Technical Summary

Problems solved by technology

If the opportunity is missed, it will greatly affect the authenticity and validity of the results of genetic experiments

[0004] In the traditional genetic experiment process, it is necessary to observe the growth and development of Drosophila artificially, obtain the eclosion time of Drosophila in time, and pick the female Drosophila within 10 hours of eclosion. Long length, too many samples, and different strains of fruit flies involved in the experiment brought difficulties to the experiment

The specific difficulties are as follows: 1. Drosophila is a living thing, and its growth and development are affected by its own genetic factors and environmental factors. There are many Drosophila strains participating in genetic experiments, and their developmental processes are different. The interference between experimental groups is large, and the experimental results are prone to appear. Deviation; 2. The number of experimental groups is large, and the number of fruit fly culture bottles is huge. It is time-consuming and laborious to observe the developmental state of fruit flies in many fruit fly culture bottles by manual methods. Omissions affect the experimental results; 3. Experimenters repeatedly enter and exit the laboratory for a long time, interfering with the normal progress of other experiments in the laboratory, making laboratory management difficult and experimental safety not guaranteed

[0005] At present, computer vision technology is becoming more and more mature, and wireless data transmission technology is changing with each passing day. The use of video sensors to obtain dynamic images and target analysis of images has played an important role in the fields of medicine, traffic monitoring, and astronomical observation. Related technologies have also been applied to insects. Observation-related research, such as the invention patent with application number 201310052396.X, uses traps to lure pests to touch the power grid, causing current changes, and is used to count pests. However, at this time, the pests have died due to the high voltage of the power grid and cannot be used for subsequent Experimental research on genetics; the invention patent with application number 201911305779.7 discloses a method for tracking and observing Drosophila expressing red fluorescent protein in the eyes, but there is no evidence that this method can be used in traditional genetics experimental teaching in a conventional sense Research on the growth, development and eclosion of wild-type fruit flies and non-transgenic fruit flies of various mutant strains used in the study; the invention patent with application number 201310574185.2 uses solar power supply devices, fruit fly trapping devices, and control and monitoring devices to realize real-time monitoring of fruit flies in the wild. Monitoring, this method uses outdoor natural light to monitor a large number of adults, but cannot complete the monitoring work of fruit flies in the dark environment of the incubator in the laboratory

The above technologies are all aimed at a single fruit fly collection-observation device, and the observation objects are only adult fruit flies or other insects. They observe the state of fruit flies in a large number of fruit fly cultivation devices in an orderly manner, but cannot find and report the cultivation in time Drosophila in a bottle completes eclosion from pupa to adult

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