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Application of CY-09 in preparation of medicine for resisting joint prosthesis wear particle induced osteolysis

A technology of CY-09 and wear particles, applied in the field of biomedicine, can solve problems such as unclear mechanism of action, achieve the effect of delaying effectiveness, broad application prospects, and delaying the degree of skull osteolysis

Pending Publication Date: 2021-01-22
SHANGHAI FIRST PEOPLES HOSPITAL
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  • Claims
  • Application Information

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Problems solved by technology

The invention patent with publication number CN111419842A discloses the important role of CY-09 in the treatment of nonalcoholic steatohepatitis, but its mechanism of action in periprosthetic osteolysis mediated by wear particles is still unclear, so this study is expected to Expansion of new clinical applications of CY-09

Method used

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  • Application of CY-09 in preparation of medicine for resisting joint prosthesis wear particle induced osteolysis
  • Application of CY-09 in preparation of medicine for resisting joint prosthesis wear particle induced osteolysis
  • Application of CY-09 in preparation of medicine for resisting joint prosthesis wear particle induced osteolysis

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Embodiment 1

[0031] Example 1 In vivo and in vitro studies of CY-09 inhibiting osteolysis induced by artificial joint wear particles (1) Materials and methods

[0032] 1. Cell Culture and Collection of Clinical Specimens

[0033] The mouse macrophage cell line Raw264.7 was purchased from the Institute of Biochemical Cells, Chinese Academy of Sciences, using Hyclone high-glucose medium (containing 10% fetal bovine serum and 1% penicillin and streptomycin). After the approval of the Ethics Committee of Shanghai First People's Hospital and the informed consent of the patients, the boundary membrane tissue samples of patients with artificial hip joint prosthesis loosening, synovial tissue samples of patients undergoing joint replacement due to hip arthritis, and related case follow-up data were collected .

[0034] 2. Western Blot

[0035] The processed cell samples or tissue samples were added to an appropriate amount of pre-cooled RIPA lysate containing protease inhibitors to lyse for 30 m...

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Abstract

The invention discloses an application of CY-09 in preparation of a medicine for resisting prosthesis wear particle induced osteolysis. Experimental results show that high-concentration CoCrMo wear particles can induce the increase of the level of reactive oxygen species (ROS) in macrophages, activate intracellular inflammasomes and secondary pyroptosis, generate various inflammatory factors including IL-18, IL-1[beta] and HMGB1, and promote the formation of an extracellular inflammatory microenvironment. After the CY-09 is added, the content of the inflammasomes in the macrophages stimulatedby the high-concentration wear particles is remarkably inhibited, and then the degree of macrophage inflammatory factor exocytosis can be reduced. In addition, the CoCrMo wear particles are implantedinto a model for skull subcutaneous induction of skull dissolution, then subcutaneous injection of the CY-09 is carried out to apply effective intervention, and in-vivo experiments further prove thatthe CY-09 has a remarkable inhibition effect on the osteolysis induced by the wear particles.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of CY-09 in the preparation of anti-artificial joint wear particle-induced osteolysis drugs. Background technique [0002] Artificial joint replacement is by far the most effective means of treating advanced osteoarthritis (including advanced osteoarthritis, femoral head necrosis, rheumatoid arthritis, etc.). However, different artificial joint interfaces, including metal-metal, metal-ceramic, etc., will inevitably produce some nano-scale wear particles due to friction factors in the body, and the accumulation of these foreign body particles in the body can be harmful to macrophages, fibroblasts, etc. And osteoblasts and other cells produce irreversible damage. Studies have shown that artificial joint-derived wear particles are one of the main factors causing periprosthetic osteolysis. On the one hand, wear particles induce inflammation-related cells to secr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/426A61P19/08A61P29/00
CPCA61K31/426A61P19/08A61P29/00
Inventor 薛松马金忠桑伟林朱力波王聪陆海明徐以明钟毅鸣
Owner SHANGHAI FIRST PEOPLES HOSPITAL
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