SNP molecular marker closely linked with solanum melongena fruit length major QTL locus and application
An eggplant fruit and molecular marker technology is applied in the field of molecular genetics and breeding to achieve the effects of shortened selection time, high linkage and reliability, and simple and fast detection.
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[0037]Obtaining of SNP molecular markers closely linked to the major QTL sites of eggplant fruit length:
[0038]A. Use Zhejiang purple red line eggplant ‘1838’ and green oval eggplant ‘1815’ to obtain F1, F1Obtained 131 F strains by selfing2Segregated groups are the research objects;
[0039]B. For F2Individual plant, measure the fruit length of 3 normal commercial fruits for each individual plant, take the average value as the fruit length value of the individual plant, then select the first 18 extremely long plants and 18 extremely short plants according to the fruit length value, using the improved CTAB Method to extract DNA from extreme individual plants and parents;
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