Supercharge Your Innovation With Domain-Expert AI Agents!

SNP molecular marker closely linked with solanum melongena fruit length major QTL locus and application

An eggplant fruit and molecular marker technology is applied in the field of molecular genetics and breeding to achieve the effects of shortened selection time, high linkage and reliability, and simple and fast detection.

Active Publication Date: 2021-01-22
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above-mentioned technical problems existing in the selective breeding process of eggplant fruit length, the present invention provides a SNP molecular marker closely linked to the main effect QTL site of eggplant fruit length, a detection method and an application, which has the function of reducing breeding The characteristics of workload, shortening breeding time and improving breeding efficiency

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SNP molecular marker closely linked with solanum melongena fruit length major QTL locus and application
  • SNP molecular marker closely linked with solanum melongena fruit length major QTL locus and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037]Obtaining of SNP molecular markers closely linked to the major QTL sites of eggplant fruit length:

[0038]A. Use Zhejiang purple red line eggplant ‘1838’ and green oval eggplant ‘1815’ to obtain F1, F1Obtained 131 F strains by selfing2Segregated groups are the research objects;

[0039]B. For F2Individual plant, measure the fruit length of 3 normal commercial fruits for each individual plant, take the average value as the fruit length value of the individual plant, then select the first 18 extremely long plants and 18 extremely short plants according to the fruit length value, using the improved CTAB Method to extract DNA from extreme individual plants and parents;

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of molecular genetic breeding, and particularly discloses an SNP molecular marker closely linked with an solanum melongena fruit length major QTL locus, adetection method and application. The SNP molecular marker is located on the 78393511st basic group of an solanum melongena chromosome 3, and the 78393511st basic group is T or A; the SNP locus is converted into a KASP marker; a KASP primer is a forward primer, F-1: GAAGGTGACCAAGTTCATGCTCGACAATGTTCACGGAACAAGT, as shown in SEQ ID NO: 3, a forward primer, F-2: GAAGGTCGGAGTCAACGGATTCGACAATGTTCACGGAACAAGA, as shown in SEQ ID NO: 4, and a reverse primer, R: AGATATACTAGGATTTGGTTTC, as shown in SEQ ID NO: 5. The molecular marker has the characteristics of reducing the breeding workload, shortening the breeding time and improving the breeding efficiency.

Description

Technical field[0001]The present invention relates to the technical field of molecular genetic breeding, in particular to a SNP molecular marker closely linked to a major QTL site of eggplant fruit length, a detection method and application.Background technique[0002]Eggplant (Solanommelongena L., 2n = 24) is an important vegetable crop of the Solanaceae (Solanaceae) genus (Solanum), which has important agricultural economic value and health care effects; my country is the first producer of eggplants, and the annual output of eggplants accounts for About 60% of the world's output; my country's eggplant varieties have obvious regional differences due to different ecological environments and consumption habits, especially in fruit length, which is controlled by complex quantitative genetic trait loci (QTL). Using traditional breeding methods to breed eggplant fruit length traits is more time-consuming and laborious. Generally, the fruit length of commercial eggplants can only be known ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156Y02A50/30
Inventor 魏庆镇包崇来胡天华胡海娇王五宏汪精磊严亚琴
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More