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Method for efficiently inducing antibody to hepatitis virus, antibody and detection system

A hepatitis B virus and antibody technology, applied in the direction of virus/phage, virus antigen component, virus, etc., can solve the problem of unable to prevent HBV infection, and achieve the effect of inhibiting infection

Pending Publication Date: 2021-01-22
NAT INST OF ADVANCED IND SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, recent reports have reported cases where HBV infection was not prevented even though the HBV vaccine prevented the onset of hepatitis B

Method used

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  • Method for efficiently inducing antibody to hepatitis virus, antibody and detection system
  • Method for efficiently inducing antibody to hepatitis virus, antibody and detection system
  • Method for efficiently inducing antibody to hepatitis virus, antibody and detection system

Examples

Experimental program
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preparation example Construction

[0150] [Preparation of Antibody]

[0151] The monoclonal antibody of the present invention can be produced, for example, by methods known to those skilled in the art (hybridoma method, phage display method, etc.) described below.

[0152] Antibody-producing B cells can be obtained from the spleen, lymph nodes or peripheral blood of antigen-immunized mice, and antibodies can also be obtained from immunized mice by preparing hybridomas or obtaining cDNA.

[0153] Specifically, for the hepatitis B virus antigen having sugar chains on the HBs glycoprotein (PreS1, PreS2 or S region), the HBs glycoprotein having the structure of the above formula [1], [2] or [3] The monoclonal antibody to the hepatitis B virus antigen or the hepatitis B virus antigen described in the item "HBs glycoprotein" can be administered to a mammal by Hepatitis B virus antigen, a hepatitis B virus antigen derived from HBs glycoprotein having the structure of the above formula [1], [2] or [3], or the hepatiti...

Embodiment

[0213] Hereinafter, the present invention will be described more specifically by showing examples, but the present invention is not limited to these examples.

[0214] In order to distinguish large spherical particles from SVP, we first focused on the sugar chain modification on HBV particles, and analyzed the sugar chain attachment and sugar chain structure of each HBs antigen. A particle fraction containing HBV particles was prepared from pooled sera of HBV-infected patients using the ultracentrifugal concentration method. Particle components were used in various studies after inactivation by heat treatment at 60 °C overnight. In the presence of 0.4% SDS and a reducing agent (0.2M dithiothreitol; DTT), the concentrated HBV particles were heat-treated at 95°C for 5 minutes, and analyzed by SDS-PAGE (Fujifilm Wako Pure Chemical Industries, Ltd. Wako Pure Chemical Industries, Ltd.) SuperSep TM Ace, 10-20%) developed and fixed for silver staining ( figure 2 ).

[0215] Sim...

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Abstract

To provide an examination system whereby an antigen having a sugar chain added thereto can be recognized in Dane particle of hepatitis B virus (HBV), and a neutralizing antibody that recognizes an antigen having a sugar chain added thereto and exhibits an inhibitory effect on infection. By clarifying that Dane particle relates to a specific sugar chain structure, it becomes possible to construct anovel detection system for hepatitis B virus particles which show infectivity, i.e., contain a nucleic acid. Further, it becomes possible thereby to provide a neutralizing antibody that recognizes anantigen having a sugar chain added thereto and exhibits an inhibitory effect on infection.

Description

technical field [0001] The present invention relates to hepatitis B virus antigen and its production method, vaccine composition containing hepatitis B virus antigen, anti-hepatitis B virus antibody and nucleic acid encoding the antibody, detection system of hepatitis B virus, and hepatitis B Pharmaceutical composition for viruses. Background technique [0002] Hepatitis B virus (HBV) is a hepadnavirus with incomplete double-stranded DNA, and is a virus that specifically infects the human liver. Due to HBV infection, it is possible to develop acute hepatitis B, chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma, which is one of the major health problems. It is believed that about 1% of the population (about 1.1-1.4 million) of the population in Japan currently have hepatitis B virus (HBV) holders, and about 5% (350 million people) of the world are infected (WHO report) . [0003] Hepatitis B is treated with immunotherapy based on interferon (antiviral drug) ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/02A61K39/29A61P31/20A61P37/04C07K16/00C07K16/08C07K16/18C07K16/46C12N15/13C12P19/00G01N33/53G01N33/566G01N33/576A61K39/395
CPCA61P31/20A61P37/04C07K16/082C07K2317/34C07K2317/76G01N33/5764G01N2400/02G01N33/56983C07K14/005C12N2730/10122C12N2730/10134A61K39/12A61K2039/575A61K2039/55566A61K2039/6081A61K39/292A61K2039/505C07K2317/56G01N33/5761
Inventor 成松久安形清彦梶裕之久野敦佐藤隆千叶靖典栂谷内晶清水弘树曾我部万纪我妻孝则沟上雅史是永匡绍田尻和人小泽龙彦
Owner NAT INST OF ADVANCED IND SCI & TECH
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