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Method for rapidly removing ethanol in chloroform

A chloroform and ethanol technology, applied in the field of biochemistry, can solve problems such as failure to find and remove chloroform ethanol, poisoning of laboratory personnel, pollution of laboratories, etc., and achieve the effects of simple equipment and reagents, reduced experimental equipment, and reduced toxic effects.

Inactive Publication Date: 2021-01-29
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It can be seen that the liquid separation distillation method has many steps, is time-consuming and laborious, and it takes 6-9 hours to prepare an ethanol-free chloroform
Moreover, during the longer experiment process, especially the volatilization and decomposition of chloroform during the high-temperature distillation process polluted the laboratory, which would have a strong toxic effect on the experimenters.
[0005] At present, in addition to the above-mentioned liquid distillation method, no other method has been found to remove ethanol in chloroform

Method used

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  • Method for rapidly removing ethanol in chloroform
  • Method for rapidly removing ethanol in chloroform
  • Method for rapidly removing ethanol in chloroform

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Obtaining ethanol-free chloroform:

[0024] (1) Activated alumina chromatographic columns are commercially available, such as figure 1 As shown, the outer diameter of the glass chromatography column is 350mm, the effective length is 250mm, there are 4 triangular glass plates at the bottom, a glass piston, and a glass stopper on the top inner ground.

[0025] Put the chromatographic column on the iron stand, close the glass stopcock, spread 2 layers of filter paper lightly wetted with chloroform on the bottom of the column, then slowly put the analytically pure activated alumina particles into the 350mm×250mm chromatographic column, and then Spread 2 layers of filter paper on top of the alumina particles.

[0026] (2) Determine the amount of activated alumina to achieve the best removal effect by setting the gradient experiment:

[0027] ①According to the above method, set up the activated alumina chromatography column filled with 6 gradient experiments; among them, th...

Embodiment 2

[0032] Utilize the above-mentioned chloroform that removes ethanol to be used for soil microbial biomass determination of Pinus sylvestris pine plantation:

[0033] (1) Preparation of soil samples

[0034] The experiment of this example uses 6 fresh soil samples of 0-10cm taken from Pinus sylvestris sylvestris plantation, and the sample numbers are 1, 2, 3, 4, 5, 6. Each soil sample takes by weighing 12 parts of 20g fresh soil and puts it into a 50ml glass beaker, wherein, 3 parts are used for fumigation without ethanol chloroform after the method of the present invention is processed, and 3 parts are used for fumigation by commercially available alcohol-containing chloroform as a contrast, 3 parts were used to prepare chloroform fumigation by separation distillation as described in the background technology for fumigation as a control, and 3 parts were used as a blank control without fumigation.

[0035] (2) Fumigation

[0036] Pour 25ml of the alumina and chloroform prepar...

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Abstract

The invention relates to the field of biochemistry, in particular to a method for rapidly removing ethanol in chloroform. The chloroform to be treated is stranded and adsorbed through alumina particles, and the chloroform with the ethanol removed is obtained. Compared with a liquid separation distillation method commonly adopted at present, the method is simple and rapid to operate, heating distillation is not needed, the volatilization and decomposition of chloroform in the experiment process are reduced, and pollution to a laboratory and poison to experimenters are greatly reduced.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a method for rapidly removing ethanol in chloroform. Background technique [0002] Soil microorganisms are an important part of the ecosystem and play a key role in maintaining ecosystem biodiversity, organic matter decomposition, and nutrient transformation. Microbial biomass carbon, nitrogen, and phosphorus are important indicators for studying soil microorganisms and ecosystem material cycles. [0003] The most commonly used method for the determination of soil microbial biomass is the chloroform fumigation extraction method. The key point of the method is to place the soil and ethanol-free chloroform in a vacuum desiccator, and keep the vacuum state after the chloroform is boiled through an air pump. After soil samples were fumigated with chloroform (1-3 days), the dead cells of soil microorganisms were lysed, releasing microbial biomass carbon, nitrogen and phosphorus. Then, b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 赵琼艾桂艳曾德慧
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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