Experimental method of paeonol used as antibacterial agent against Aspergillus flavus
A kind of Aspergillus flavus, experimental method technology, applied in the direction of material excitation analysis, fluorescence/phosphorescence, instruments, etc., can solve the problems of high probability of carcinogenicity, strong toxicity, threat to human health, etc., and achieve the effect of high reliability and convenient operation
Active Publication Date: 2021-11-05
HENAN UNIVERSITY OF TECHNOLOGY
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At the same time, Aspergillus flavus produces aflatoxin, which is highly toxic and has a high chance of causing cancer, which seriously threatens human health.
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Embodiment 1
[0039] A kind of experimental method that paeonol is used as aspergillus flavus antibacterial agent comprises the following steps,
[0040] Step 1, prepare medium, prepare two groups of medium groups, respectively the first medium group and the second medium group, the first medium group is SDA (Sabouraud dextrose agar medium), the second medium group is SDB (Sabouraud Dextrose Broth);
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Abstract
The present invention relates to paeonol, in particular to an experimental method for using paeonol as an antibacterial agent for Aspergillus flavus, introducing paeonol as an inhibitor of Aspergillus flavus, which has the advantages of high efficiency, non-toxicity, environmental friendliness, economical applicability, etc. The effect of paeonol concentration on Aspergillus flavus mycelia can quickly determine whether paeonol can inhibit Aspergillus flavus, and can also obtain the low-value MIC of paeonol inhibitory concentration. Accurately judging the antibacterial mechanism of paeonol can not only confirm the inhibitory effect of paeonol on Aspergillus flavus, but also provide a basis for the selection of application occasions. Quality content detection, β‑1, 3‑glucan detection, can comprehensively judge the antibacterial mechanism, carry out verification through verification test, can confirm the effectiveness of paeonol as a flavus bacteriostatic agent, and form a complete Experimental system, high reliability.
Description
technical field [0001] The invention relates to paeonol, in particular to paeonol used as an antibacterial agent for Aspergillus flavus. Background technique [0002] Paeonol is an active ingredient of traditional Chinese medicine extracted from the root bark of Moutan. This drug has anti-inflammatory, labor pain, antipyretic and anti-allergic effects. At the same time, paeonol has broad-spectrum antibacterial properties, and has inhibitory effects on both bacteria and fungi. However, there are few reports on paeonol's inhibition of Aspergillus flavus, and the antibacterial mechanism has not yet been proposed. [0003] Aspergillus flavus, a common fungus, is extremely prone to contamination during postharvest storage and food processing. As an opportunistic pathogen, it can also cause aspergillosis in humans and animals. At the same time, Aspergillus flavus produces aflatoxin, which is highly toxic and has a high chance of causing cancer, which seriously threatens human hea...
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IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6439
Inventor 李倩朱晓嫚赵颖
Owner HENAN UNIVERSITY OF TECHNOLOGY
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